Cloning Of Psmapk1, Pscdc2, Psfuz7 And Functional Characterization Of Psmapk1 From Puccinia Striiformis F.sp. Tritici

In this paper, some explanations have been given about the transcriptional profilings of mitogen-activated protein kinase (PsMAPK1), cell division cycle 2 (PsCdc2) and mitogen-activated protein kinase kinase PsFUZ7. The differentiation of PsMAPK1 and PsFUZ7 amino acid sequences in stripe rust CYR23, CYR25, CYR29, CYR31 CYR32 and CYR33 were also reported. In addition, we described the gene potential function of PsMAPK1 by complementation of the Fusarium graminearum mutant and Magnaporthe grisea mutant.Mitogen-activated protein kinase MAPK1, cell division cycle Cdc2 and mitogen-activated protein kinase kinase FUZ7 from Puccinia striiformis f.sp. tritici (Pst) were firstly isolated through screeming the DNA library. We tentatively designated these three gene as PsMAPK1, PsCdc2 and PsFUZ7. The cDNA sequence of PsMAPK1 is 1834 bp contain a 1227 bp open reading frame and encoded a putative protein composed of 408 amino acids. The DNA sequence of PsMAPK1 is 2343 bp, comprised of 8 extrons and 7 introns. The cDNA sequence of PsCdc2 is 1395 bp contain a 885 bp open reading frame and encoded a putative protein composed of 294 amino acids. The DNA sequence of PsMAPK1 is 2279 bp, comprised of 8 extrons and 7 introns.The cDNA sequence of PsFuz7 is 2093 bp contain a 1260 bp open reading frame and encoded a putative protein composed of 419 amino acids. The DNA sequence of PsMAPK1 is 2661 bp, comprised of 8 extrons and 7 introns. Real time RT-PCR analysis showed that PsMAPK1 was up-regulated at early stage of infection, the maximum induction occurred at 24 hpi, at which transcripts were 5.32 fold over that in urediniospore. The accumulation of transcripts decreased obviously after 36 hpi. The maximum induction of PsCdc2 occurred at 12 hpi, the transcripts were 1.62 fold over that in urediniospore. The accumulation of transcripts decreased steadily after 12 hpi. PsFuz7 was up-regulated at early infection(before 12 hpi), The maximum induction were 1.95 fold over that in urediniospore., which occurred at 12 hpi. The induction at 6 hpi was 1.79 fold over that in urediniospore, and after 18 hpi, the induction were all lower than that in urediniospore. Sequences analysis of PsMAPK in different stripe rust race, CYR23, CYR25, CYR29, CYR31, CYR32 and CYR33, showed that there was no obvious differentiations, but there might be 9 changeable mutant locations. PsFuz7 also had no obvious differentiations, but there might be 12 changeable mutant locations. In addition, PsMAPK1 can complement the the Fusarium graminearum mutant and Magnaporthe grisea mutant. PsMAPK1 might be essennntial for pathogenicity and infectious growth in stripe rust fungi.