Font Size: a A A

Influence Of Glycinebetaine And Proline On Physiology Characteristic Of Salt-stressed Processing Tomato Plants

Posted on:2010-01-14Degree:MasterType:Thesis
Country:ChinaCandidate:J Y LiuFull Text:PDF
GTID:2193330338952885Subject:Vegetable science
Abstract/Summary:PDF Full Text Request
The experiment was conducted in greenhouse of Agriculture Science Department of Shi hezi University. Four different cultivars of processing tomato (Shihongsanhao;Hongba;87-5;K20)were tested. In this paper, the physiological response was studied to processing tomato varieties seedling growth when different types of processing tomato varieties were under saltstress, And so the ameliorative effect of proline and glycinebetaine on seedling.The effects of different processing tomato genotypes and proline and glycinebetaine concentration when they were under salt stress was also explored. Such as effects on processing tomato plant height,fresh weight and dry weight,chl content, osmotic adjustment substance content, Na+and K+content,anti-oxidation enzyme activity and anti-oxidation substance content and so on.The results indicated that:1 The growth of processing tomatos seedlings is promoted by low salt(50 mmol·L-1NaCl) stress,and the fresh and dry weight of the shoot and root part of seedlings and plant height are increased in the seventh day,but he fresh and dry weight of the shoot and root part of seedlings and plant height are decreased in the fourth day.high salt (100 mmol·L-1 NaClå'Œ150mmol·L-1 NaCl)stress inhibits the growth of processing tomatos seedling, and the fresh and dry weight of the shoot and root part of seedlings and plant height are reduced obviously. Under the stress of Nacl, the content of chl of processing tomato seeding leaves was reduce with the NaCl content,The fresh and dry weight of the shoot and root part of seedlings and plant height and the content of chl can be increased by adding exogenous proline and glycinebetaine in the same NaCl content stress.2 In a certain concentration range, Nacl will cause tomato's normal physiological processing disorder; Under the stress of Nacl, the soluble sugar content, Pro content, MDA, membrane permeability of tomato seeding have shown an upward trend, and have increased with the time and concentration. The MDA and membrane permeability could be reduced by adding exogenous proline and glycinebetaine, but Pro content and soluble sugar content would be increased.,in the same time significantly improve Na+content of tomato seedlings, reduce K+ content in the 7d; The same of the tend in 7d and 14d,but the degree of damage and catabatic of proline and glycinebetaine was high than the 14d.3 Under the stress of Nacl, the activity of SC,POD,CAT,APX and content of ASA of tomato seeding leaves have shown an downward trend, compared with standard treatment(CK), the content of antioxidants and activity of antioxidase of leaves which were treated by proline and glycinebetaine keep in high level.In the 7d of NaCl stress, the activity of contradict oxidation enzyme and contradict oxidation substance was reduce with the increase of NaCl content; he activity of contradict oxidation enzyme and contradict oxidation substance was continue reduce,except of the deal with of ck and accede to proline and glycinebetaine in ck was increase.4 Proline and glycinebetaine had concentration effect, in this experiment,1-5mmol·L-1 betaine and 1mmol·L-1 Pro were able to reduce and alleviate salt stress harm to tomato plants,5mmol·L-1 betaine had significant role in mitigation.10mmol/1 praline would inhibit plant growth and even lead to plant death.5 There were difference between tomato varieties, the soluble sugar accumulation of K20 was higher than Hongba, the level of 87-5 and Shihongsanhao between them. K20's membrane injury and Na+ accumulation was also higher than the Hongba. According to this study, Hongbahas best salt-tolerant,K20 was worst, and 87-5 and Shihongsanhao between the two.
Keywords/Search Tags:salt stress, proline, glycinebetaine, tomato seedlings
PDF Full Text Request
Related items