Lily Vitro Bulb Formation

Bulb1ct Formation in Vitro of LililInlPostgraduate student: Deng Liu-HongTLtor professor: Hao Bing-Zhong and Wu Ji-Lil1(South China University of Tropical Agric[lture;Chinese Academy of Tropical Agricutural Sciet1ce)AbstractBy in vitro culture. the scales of LililI))1 bulb are able to produce man}' scale bulblets,tioln 'tthich the plal1tlets are derived in contil1uous culture. The plal1tlets produce bulbletson their base \\.hich can be used fOr comlnecial culture. Tl1is stud}' \\'as n1ade to sho'" thethctors int1uencing the formation in vitro of plantlets and bulblets. tl1e thctors includi11gJasl11ol1ic acid (JA) applicatiol1 in especial.Tlle optimal n1edium tbr lililInI tissue culture is as t1lo\":MS + 0.5111g/L BA +0. lmg/L NAA or MS + 0.5l11g/L BA + 0.5mg/L NAA.Our study sho'"ed bulblet tbrmation ratio tiom scales depended on liliIII)Ix'ariaties.The scales as explants tiom the outside and n1iddle of tl1e bulb tel1ded toproduce more bulblets in comparision with the inl1er scales. The scales produced morebulblets in their base al1d middle than that in the parts distal tiom tl1e base.JA llad i11hibitol3' ettbcts ol1 buIblet il1itiatiol1 ti.ol11 Li/ilIIII scale al1d tl1e l1igI1er t11eJA concentrations. the stronger the inhibition. The JA concentratio11s et1kcti\.e in tl1eilillibition \"ere l0~l00n1g/L. It is assumed that the bulblet initiatiol1 depel1ds on therelease of tl1e dormal1cy of the scales and JA prevents them t\om dorn1al1c}' release.JA accelerated the bulblet swelling in plant1ets deri\.ed tiom scale bulblets. The JAcollce11trations 0.05~501ng/L wele effective i11 acceleratio11. Tl1e l11ost e11bctix.e 1\'ere0. l ~2.5lllg/L JA that resulted in the formation of the bulblets x'itl1 tiesl1 x"eigl1t835~ 1022lllg/per bulb1et. whi1e the bu1blets in control lneasured 476111g/per bu1blet. lt isassul11ed that JA l1as a inhibitory eftbct on plal1tlet gro''1l1 al1d lead it to turn to28production . when the food accumlation in bulblets is accelerated by JA.Light microscopical observation was made of the scale bulblet initiation from the scales as explants and the bulblet formation from plantlets.