The Butterfly Orchid Into The Study Of The Physiological And Biochemical Mechanism Of Floral Induction And Differential Gene Expression

The effect of temperature on the bloom induction of phalaenopsis was studied in this paper. The changes of the level of three endogenous phytohormones (GA3,IAA,ABA) in phalaenopsis leaves were ananlysized by the CZE method. At the same time we studied the genes' differential expression of phalaenopsis by the DD RT-PCR strategy.The results of our research show that the optimum temperature for inducing phalaenopsis to bloom is 25/20 ℃ (day/night). Phalaenopsis treated with the 22/1 6℃ and 23/1 8℃ bloomed almost at the same time with those cultivated under 25/20℃, but a few of them failed to flower. The same inducing effect can be achieved by moving the phalaenopsis to the natural temperature after treated under25/20癈 for 30 days. The GAs-treated plants didn't bloom earlier than the ones cultivated under natural temperature, but had longer inflorescence. Phalaenopsis treated with 30/28 C didn't spike at all.There is a notable increase on the level of endogenous GAs 20-30days before spiking, and the high level of endogenous GA3 had been maintained for a short term after the spiking, suggesting that there is a relationship between the level of endogenous GAs and the spiking and elongation of phalaenopsis'' inflorescence. The level of endogenous IAA and ABA didn't change much before and after spiking.Take advantage of the DD RT-PCR technics to explore the differetial expression of the gene of individuals treated under 25/20℃ (day/night) for 10 days. We had got 22 differenttial cDNA fragments, and exammed the sequence of 5 of them. This part of work is the base for future work of our lab.