Dietary Selenium Rex Rabbit Production Performance, Physical And Chemical Indicators And Gpx Gene Expression

A experiment was conducted to determine the appropriate amounts of Selenium(Se) in rex rabbit diet, 72 50 to 55-days-old rex rabbits from a primary breeding farm associated Vc-II in the Quartermaster University of P.L.A were selected, and randomly divided into 6 groups, each group was further divided into 3 subgroup of 4. The whole experiment period was 60 days. The animal in control group was fed a basic diet without any Se, and the experimental groups was fed the basal diet supplemented with 0.1, 0.25, 0.4, 0.55, 0.7mg/kg Na2SeO3 , respectively. The rex rabbit were weighed, and blood sampled were taken from heart at 20th, 40th and 60th day of experiment period in order to detect and analyze the performance and physiochemical parameters. In addition, the expression amounts of mRNA from GPX in livers of rex rabbits after 60 days was detected with RT-PCR set by an inner parameters of p-actin.The results showed : (1) Selenium supplementation could increase daily gain of rex rabbit. The result was different in the other periods. In first 40 day, there was no significant difference in daily gain between groups, however, the group with 0.4mg/kg addition of Se had a slightly higher daily gain than that of the other groups. During 40-60 days , there was no difference in daily gain and the group with addition of 0.25mg/kg was optimal. From the item of feed/gain, during 0~40d, the conclusion could be drawn that the group with 0.4mg/kg was minimum. During 40~60d, thegroup of 0.25mg/kg was highest than that of the other groups. Hide surface area of the group of 0.4mg/kg was larger than those of the other experimental groups. (2) There was no significant difference among groups in total protein and serum album. However, the 0.4mg/kg group had slightly higher content than thost of the other groups at end of 40d. During 40~60d, there were significant difference among groups with the group of 0.25mg/kg supplement being the highessst. Serous T3 in 0.4mg/kg group was highest during 0~40d, and when 40~60d, the group with 0.25mg/kg accession was highest than the other groups. According to GPX in blood or tissue, significant differences were found among groups, and the 0.4mg/kg group showed highest activity than the other groups. Sediment amount of Selenium accession no matter what in blood or tissue increased with growth of Selenium accession, and remarkable difference was showed compare to the control group. (3)Analyzing from the expression amounts of GPX, every group had respectively increased 64%, 82%, 96%, 95% and 93% of expression amounts about GPX in comparing to the control group. To sum up, it can be concluded that the most appropriate Selenium accession to rex rabbit was that 0.4mg/kg was optimal during 0~40d and that 0.25mg/kg was optimal during 40~60d.