Ramie Induced Apomixis With Chemical To Kill The Male Test

It is observed the induced apomixis ramie, identified somatic cell chromosome ploidy of the first generation of induced apomixis plants (AF1) and used chemical agents to research the anthericide test on ramie.1 .Using two kinds of agents induce ramie apomixis. It is researched the ramie sacs by the modified whole stain-clearing method and paraffin method. The results show that the treatment of agents caused some abnormal phenomena during the embryologenesis of ramie. Three antipodal cells are observed in the pole of chalaza after the embryo sac is induced for four days. The middle antipodal cell with an egg-like shape is big, and the other two antipodal cells with synergid-like shape close the egg-like antipodal cell. There are two polar nucleuses in the middle of the embryo sac develops plumply, while the uninduced embryo sac atrophied and deformed. The embryo and the endosperm maybe respectively originate from the egg-like antipodal cell, and secondary nucleus autonomously divided without pollination. Because of the induce efficiency, the type of ramie embryogenesis induced apomixis has not known yet, so more researches are needed.2. The somatic cell chromosome of AF( are observed. In 174 AF| plants, there are 20 plants of haploid plant (11.5%), 34 plants of diploid plants (19.5%), 38 plants of aneuploid plants (21.8%) and 82 plants of mixoploid plants (47.1 %). The alterative range on the number of plant chromosome is between 14 and 28 commonly, and lay behind chromosomes, non-equal and mutiple polar mitosis are observed. It may be the reason of forming aneuploid plants and mixoploid plants.3. It uses two kinds of chemical anthericides in ramie. The results show that Cresylureas is optimal in the concentration of 500 and 800 ppm, and the antheridia rate upto 98.0% and 100%, the artificial pollinating fertility upto 53.1% and 60.9% respectively. Eth is an efficient chemical anthericide of ramie, but it is harmful to female flower mostly.