Diamondback Moth Resistance To The Molecular Mechanism Of Discussion And Analysis

Plutella xylostella L. (Lepidoptera) is the main pest of crucifer. The resistance management of Plutella xylostella(L.) is a hard and serious problem in the world. However, up to now, most study is in physiology and biochemistry, little is known about the genetic and molecular bases. Themechanism of resistance is very complex which refers to the change of behavior, enzymes in metabolizability and insecticide targets.In this study, We used susceptible strain and dimehypo-resistance strain , deltmethrin-resistance strain , cartap-resistance strain of diamondback moth as material and studied the mechanism of insectcide resistance by electrophysiology, radioactive immunity experiment and two-dimentional electrophoresis.Followed are our main results:1.Analysis the relation of insectcide resistance with mariner transposable element by normal ways of molecular biology. We found a new type of mariner transposable element, but it is not relation to resistan.2. Analysis the effects of dimehypo and cartap to the spontaneous active potential of central nerve of dictyoptera by electrophyological method.Dimehypo and cartap can increase swing and frequence of the spontaneous active potential of central nerve of dictyoptera strongly.3. NAchR was separated from the head' s of Plutella xylostella It includes three subunits with the molecular weight of 421flX 38KD, 28KD. The nAchRs of dimehypo-resistance strain cartap-resistance strain have remarkable insensitivity to (3-[I125]iodotyrosyD a-Bungarotoxin.4.Two-dimentional electrophoresis was applied to find differences between susceptible strain and other three resistance strains. The differences between susceptible strain and deltmethrin-resistance are very remarkable. The diffenence between susceptible strain and dimehypo-resistance, cartap-resistance strain are similar.In conclusion, compared with susceptible strain, the nAchRs of resistance strains have remarkable insensitivity to (3-[I125] iodotyrosyl) a-Bungarotoxin. There are prominent difference between the susceptible strain and resistance strains in protein.