Different Host And Tissue Cells With The White Spot Syndrome Virus (wssv) Of The Binding Studies

This paper established a FLUOS labeled method that can be used in the binding assay between WSSV and host cells with the advantages of more convenience, quickness and less expense on the principle of specific binding between virus and receptor on the cells of host. To some extent, it proved the specific binding of WSSV and Marsupenaeus japonicus, Procamburas clarkii and Litopenaeus vannamei etc hosts by right of this method. Gill cells and hemocytes of M. japonicus have a specific binding to WSSV, but cells of muscle and hepatopancreate have not this binding. This binding reflected that receptor of WSSV on the cells of host. The binding results of different tissue cells of hosts P. clarkii and L. vannamei comparable and consistent with the binding results of M. japonicus host. Their gill cells, hemocytes and cephalothorax cuticle can binding to WSSV, but cells of muscle and hepatopancreate can not. It not only proved that the binding result of different cells of these hosts to WSSV, but also testified the binding intercommunity of WSSV and different hosts on which can find out the binding intercommunity of tissue cells to WSSV, accelerated the work for confirm and separate the receptor of WSSV.This paper extracted each host cell membrane binding to the WSSV for testified the binding activity between WSSV and different hosts and different tissue cell. The binding show that gill cell membrane, hemocyte cell membrane and cephalothorax cuticle of L. vannamei can specific binding to WSSV and cell membrane of muscle and hepatopancreate can not. In which gill cell membrane have a highest binding activity, cephalothorax cuticle membrane have a higher binding activity to WSSV and the binding activity of hemocyte cell membrane is lower. This binding result consistent with the binding result of different cells to WSSV, much more confirmed that the binding between WSSV and different tissue cells of L. vannamei. It testified that hemocyte cell membrane and cephalothorax cuticle have a restrained rolling to the binding of WSSV and gill cell membrane of L. vannamei through comparing the mutual influence of each membrane, proved that different tissue cells of L. vannamei have a same receptor to WSSV to a certain extent. P. clarkii host has a similar result that gill cell membrane and hemocyte cell membrane can binding to the WSSV, but cell membrane of muscle and hepatopancreate can not. At the same time, it analysed the influence of gill cell membrane of L. vannamei to the binding between gill cell membrane and hemocyte cell membrane of P. clarkii and WSSV, result show that the former has a inhibitionto later binding to WSSV and proved the host P. clarkii and L. vannamei have a same receptor of WSSV. To some extent, it confirmed their different tissue membrane protein consists through the SDS-PAGE.In the binding assay of different tissue membrane of Charybdis japonica to WSSV, gill cell membrane and hemocyte membrane have a specific binding to WSSV and in which the binding activity of gill cell membrane higher than hemocyte membrane, cell membrane of muscle and hepatopancreate have not this binding. Through comparing the influence of gill cell membrane and hemocyte membrane of C. japonica to binding between WSSV and gill cell membrane of L. vannamei, the result proved that the former hardly any effect to binding of later and WSSV and elementary indicated that host C. japonica and L. vannamei have a same receptor of WSSV. It just as say receptor of WSSV are not singleness, but are multi-binding location. It elementary confirmed the consist of each tissue membrane of this host by SDS-PAGE.It find out the specific binding between WSSV and cell membrane of copepod, barnacle and artemia, this binding indicated the cell membrane of three hosts have a receptor of WSSV. It as a proof for the point that copepod, barnacle and artemia belong to host range of WSSV infection and which have a positive role in illuminating the function of three host in the prevalence and break out of this disease. It confirmed the protein consists of...