Using Fluorescence In Situ Hybridization Beet Progenies

Apomixis is a means of asexual reproduction by which plants produce embryos without meiosis and fertilization; thus the embryos is of clonal, maternal origin. Beta M14 is proved to be apomictic plant. We previously analysis BAC microarrays of corollinae chromosome 9 using florescence special expression mRNA of B. vulgaris and Beta M14 BAC clone 16-M11 and 26-L15 are florescence specific expression BAC of Beta M14, both BAC clone 16-M11 and 26-L15 were in situ hybridization to monosomic addition lines(2n=2x=18+1) M14 chromosomes, they were located on the long arm of corollinae chromosome 9. the dominant factor of BAC clone 16-M11 and 26-L15 appears to hemizygous, because no equivalent region has been found in sexual Beta.Two specific expression BAC clones 16-M11 and 26-L15 were located in the close telomere region of the lone arm of a single corollinae chromosome 9. Low recombination already has been show near the telomere regions which have many repetitive DNA sequences. However, reports show that The Apomixis "locus" might be located in the low recombination regions.The large insert of BAC clones must contain many specific DNA sequences, but the hybridization signals for each of the tested BAC clones only concentrated on one chromosome regions in Beta M14 and overlapping, It means that large DNA fragments in the BAC clones were never separated during the evolutional process in Beta, and were synthetic.FISH result support our hypotheses that hemizygousity, proximity to telomere sequences, overlapping and synthetic in the two florescence specific expression BAC clones of BetaM14 may all imply BAC clones 16-MU and 26-L15 are interrelated with Apomixis, should be transform Beta as candidated gene for Apomixis, which validate its function.we also documented chromosome transmission from corollinae in M14 using genomic in situ hybridization (GISH). Only one complete corollinae chromosome was detected in Ml4, and was determined corollinae chromosome 9. We have used in situ hybridization of genomic DNA to discrimination the parental chromosomes in Beta Ml 4, suppression of cross-hybridization by blocking DNA was not necessary indicating that the investigated Beta genome contain sufficient species-species DNA enabling the unequivocal determination of genomic composition of the hybrids.In our study , the results of BAC -FISH combined with the analysis of GISH in Beta M14,indicating that present of the corollinae chromosome 9 alone was sufficient to confer apospory in Beta M14 upon celluar and molecular..