To Turn Corn Zmpis Genetically Modified Cotton And Its Resistance Preliminary Study

Phosphoinosidtide (PI) is a major phospholipid in eukaryotic cells, phospholipid signaling pathway (PI pathway) plays a key role in plant growth, development and in responses to environmental stress conditions. Phosphatidylinositol synthase (PIS) is a key enzyme in the phospholipid signaling pathway, and can synthesize Phosphoinosidtide with CDP-DG and myo-inositol. ZmPIS gene is isolated from maize, its coding production PI can be converted into many signaling factors, the factors is important in signal transduction in plants under environmental stress conditions. As an important economic crop, many works on the cotton genetic engineering have been evolved rapidly, varieties of cotton which exhibit increased resistance to pests, herbicides, pathogens, environmental stress and higher fiber quality were obtained. However, researches on the transgenic cotton is few, utilizing the mature cotton transgenic methods to obtain new elite cotton varieties that be increased resistance to drought and salinity is an important content of cotton breeding.In this paper, the naked apical meristems of cotton plantlets derived from two elite cotton cultivars Lu Mianyan19 and Lu Mianyan21 were used as the receptor of Agrobacterium mediated transformation, transform the ZmPIS gene in sense and antisense from maize into the two cultivated cottons, hoping to obtain new elite cotton that be increased resistance to drought. The main experiment results is below:(1), the naked apical meristems of cotton plantlets derived from two elite cotton cultivars Lu Mianyan19 and Lu Mianyan21 were used as the receptor of Agrobacterium mediated transformation, transform the ZmPIS gene in sense and antisense from maize into the two cotton cultivars, Southern blotting shows that the ZmPIS gene in sense has integrated with the cotton genomes. (2), analysis of the tolerance to drought stress of the transgenic cotton. T1 generation plants were selected to be exposed to osmotic stress (8%(w/v) PEG-6000) in seedling stage, including three line plants expressing ZmPIS gene in sense(L1, L2, L3), one line plants expressing ZmPIS gene in antisense(L4), and one line wild-type plants. Assay the physiological parameter and photosynthesis parameter before stress (0d) and after stress (2d, 4d, 6d). The assess of physiological and photosynthesis parameter include leaf relative water content, cell membrane demarcation, chlorophyll content, malondialdehyde (MDA) levels, proline content, total soluble sugars and net photosynthesis , stomatal conductance , transpiration rate. The results indicate that the lines expressing ZmPIS gene in sense, especially line 3, are significant better than line expressing ZmPIS gene in antisense and the wild-type control line. We could conclude that drought tolerance of the lines expressing ZmPIS gene in sense is enhanced compared with the wild-type line.In this research we transform the ZmPIS gene encoding a key enzyme in the phospholipid signaling pathway into two elite cotton cultivars Lu Mianyan19 and Lu Mianyan21 by Agrobacterium mediated transformation, and obtain the transgenic plants. The analysis of the four transgenic Lu Mianyan19 lines under osmotic stress show lines expressing ZmPIS gene in sense have enhanced drought tolerance. The transgenic plants provide a new material for further research on the roles of PI system in plant signaling, and more important it also provide germplasm for the cotton breeding in increasing resistance to salt and drought.