Brown Spot Fungi On Beet M14 Strain Resistance Induced The Role Of A Preliminary Study

Sugar beet M14 is a monosomic additional line of Beta corolliflora Zoss in Betavulgaris L., which was obtained from the intercross of the two species (Beta corollifloraZoss×Beta vulgaris L.). It was reported that Beta corolliflora Zoss was characterizedby disease resistance. So it is of great important to study the cercospora leaf spotresistance of sugar beet M14 which has No. 9 chromosome of Beta corolliflora Zoss inaddition. In this paper, cercospora leaf spot resistance of sugar beet M14 was studiedfrom two aspects:(1) According to the results of field investigation in August 2006, whether sugar beetM14 was resistant to cercospora leaf spot was determined.(2) After spraying with the Cercospora beticola Sacc. conidia suspension, enzymeactivities of peroxidase, superoxide dismutase, chinase andβ-1, 3-glucanase in leaves ofsuger beet M14 and Beta vulgaris L. were experimented, then isoenzymes of peroxidaseand superoxide dismutase were studied, and the amino acid contents in leaves were alsoanalyzed.The results showed that sugar beet M14 was characterized by moderate cercosporaleaf spot resistance compared to Beta vulgaris L.. By molecular aspect, all of theenzyme activities detected in leaves of sugar beet M14 were higher than those in Betavulgaris L., and specific bands were detected both in peroxidase and superoxidedismutase isoenzymes in infected plants of sugar beet M14. The total content of aminoacid in suger beet M14 decreased quickly after infected, while it increased in Betavulgaris L..Therefore, we can conclude that it is valuable to study sugar beet M14, which hasmoderate resistance to cercospora leaf spot.