Eggplant Rhizosphere Antagonistic Bacteria Screening And Culture Conditions

Eggplant is one of a favorite and familiar crops with people. It has a lot of area under cultivation in our country,but in recent years eggplant's serious diseases has impacted it's yield and quality ,also caused great economic losses. Therefore, we must take effective measures to carry out prevention. Control measures are being taken mainly rely on chemical pesticides,but the long-term application of pesticides on the environment will cause pollution. So,the biological control of disease on eggplant is particularly important. This experiment has screened antagonistic bacteria ,also they can make strong effect against several eggplant's disease.Isolated bacteria from healthy eggplant's rhizosphere soil of JiNing Jie village, with the preliminary-screening and again-screening of flat-screen , thirty-three stains remained,they have antagonistic effects against three kinds of eggplant's pathogens (Botrytis cinerea,Alternaria spp. I,Alternaria spp. II). With inhibitory effect of the significant degree and the stability ,genetic factors ,finally three antagonistic bacteria was left. Through orthodox morphology characteristics,the physiocal and chemical experiments and 16S rDNA sequence identified ,take the results measured with model strain's 16S rDNA sequence from EMBL / BankIT / DDBJ. Obtained develop-tree. The identification result is Q47 (1), Q69 was Bacillus subtilis, Q17 (2) was Brevibacillus pumilus.One strain Q47 (1), the integrated effect of antagonistic role is extremely clear and stable, and it's a very wide spectrum of antimicrobial, it can antagonize three supplied pathogenic bacteria , also can antagonize several other plants'pathogenic bacteria , antagonistic-rings can achieve more than ten millimeters average. It's a genuine antagoristic bacteria which has broad bacteriostasis. Thus, following research is intensive for it's preliminary culture condition. The research of culture condition about strain Q47(1) indicates us : 32℃,pH=7, keep on illuminating ,shake culture seveny-two hours is the optimal culture condition,in this period the strain has the largest yield . The research of optimal culture condition can provide advantageous accord and also make foundations for ulterior ferment works .