| Hormones are chemicals, which can adjust the body's function. They are widely used in agriculture, pharmaceutical and other industries. However, many hormones in the feed additive has been banned, because of the side effects of hormones. Chemiluminescence(CL) analysis as a sensitive and rapid detection method has attracted prople's attention in hormones analysis in recent years.The thesis consists of review and research section.In the review section, the analytical application of CL method in hormone analysis were summarized. In research report section, the CL behaviors of four sexual hormone in potassium permanganate system was studied. The experimental conditions were carefully optimized and a new flow injection CL method for the determination of four sexual hormone, namely methyltestosterone, testosterone propionate, nandrolone phenylpropionate and trenbolone, was proposed. The reaction mechanism was also briefly discussedThe research report section includes three parts.Part one, a flow injection chemiluminescence method was proposed for the determination of methyltestosterone, testosterone propionate and nandrolone phenylpropionate. It was found that chemiluminescence was produced during the oxidation of the reaction product of methyltestosterone, testosterone propionate and nandrolone phenylpropionate with sulfuric acid by potassium permanganate in acidic condition. In the presence of quinine acetate, the CL signal was enhanced significantly. Under the optimum experimental conditions The linear ranges of the method were5.0×10-8-1.0×10-5g/mL methyltestosterone, 5.0×10-8-1.0×10-5g/mL testosterone propionate and 2.0×10-7-5.0×10-5g/mL nandrolone phenylpropionate. The detection limits were 3×10-8g/mL methyltestosterone, 4×10-8g/mL testosterone propionate and 1×10-7g/mL nandrolone phenylpropionate. The relative standard deviations(n=11) were 3.0%, 4.3% and 4.0% for 5.0×10-7g/mL methyltestosterone solution, 5.0×10-7g/mL testosterone propionate solution and 5.0×10-6g/mL nandrolone phenylpropionate solution, respectively. This method has been applied to the determination of androgens in fish with satisfactory results.Part two, a new CL method was proposed for the determination of trenbolone. It was based on the CL reaction of trenbolone with potassium permanganate in the hydrochloric acid solution. The CL signal was enhanced greatly by alizarin yellow R. The CL signal was proportional to the trenbolone concentration in the range of 1.0×10-7-1.0×10-4g/mL. The detection limit was 5×10-8g/mL trenbolone. The relative standard deviation was 1.5% for 2.0×10-6g/mL trenbolone solution in eleven repeated measurements. This method has been applied to the determination of trenbolone in cattle feeds with satisfactory results.Part three, weak CL signal is observed during the reaction of potassium permanganate with flunarizine hydrochloride in acid solution. In the presence of formaldehyde, the CL signal was enhanced significantly. Based on these phenomena, the experimental conditions that affected the CL reaction were optimized and a new flow injection CL method for the determination of flunarizine hydrochloride was proposed. The CL signal was linearly dependent on the concentration of flunarizine hydrochloride in the range of 8.0×10-7-8.0×10-5g/mL. The detection limits was 8xxxxx10-7 g/mL flunarizine hydrochloride and the relative standard deviation was 2.2% for 1.0×10-5g/mL flunarizine hydrochloride solution in eleven repeated measurements. The proposed method was successfully applied to the determination of flunarizine hydrochloride in capsules. |
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