Strain Of Marijuana Unglued Function And Retting

Cannabis is an important fiber crop, its fiber has performance with durable, high added-value and it is an environment-friendly fiber raw materials, it has great potential applications. The mainly study is selection the cannabis degumming functional microbial strain and optimization of its enzyme production conditions, so as to establish a complete set of additional strains of retting process, for enhance the efficiency of degumming hemp fiber production and lay the foundation for quality.Two Function strains of degumming cannabis, HDDMM02 and HDDMG05, were isolated from the fermentation liquid of the 41# hemp retting. Two strains were identified as Bacillus licheniformis(EU723824) and Escherichia coli(EU723821) according to the morphological, physiological, biochemical characteristics and the sequencing of 16S rDNA.Single factors and response surface method was used to optimize function of two strains of the medium, and to determine the optimum conditions for enzyme production. The HDDMM02 strains grew best when cultured at 37℃with 6% konjac powder as carbon, 0.25% peptone as nitrogen source, 0.5% NaCl, 0.05% MgSO₄.7H₂O, pH 7.0, incolumn volume for HDDMM02 was 2%. The HDDMM02 strains could respectively show the highest mannanase activity as 5.89 IU/mL, the nearly 2 times before optimization. The HDDMG05 strains grew best when cultured at 37℃with 16% orange powderas carbon, 0.2% Yeast extract as nitrogen source, 0.5% NaCl, K₂HPO₄ 0.5%, 0.01% MgSO₄.7H₂O, pH 7.0, incolumn volume for HDDMM02 was 2%. The HDDMG05 strains could respectively show the highest mannanase activity as 8126U/mL, the nearly 16 times before optimization.The best laboratory conditions for retting system wes determined by determing of hemp fiber chemical composition: The strains HDDMG05 and HDDMM02 were breed largest enzyme production period, the ratio of 1:1, two inoculum strains were 0.5%, CO(NH₂)20.1%, NaCl 0.1%.The retting of hemp fiber system in the pectin, hemicellulose and lignin content were 5.318%, 2.105% and 17.954%, and without bacteria are decreased compared to three percentage points 1-3.The average rate for hemp fiber is 13.11% after add the bacteria, 11.66% before add the bacteria. The rate of hemp fiber was improved 1-1.5%, the strength of hemp fiber was improved 20-25N.