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Zigong Salt Soil Physical And Chemical Indicators Of Microbial Biomass And Diversity Of Research

Posted on:2011-12-20Degree:MasterType:Thesis
Country:ChinaCandidate:C H WangFull Text:PDF
GTID:2193360308472222Subject:Microbiology
Abstract/Summary:PDF Full Text Request
Zigong is an important salt industry and chemical production base. Salt mining residue of arid acquisition were stockpiled on ground, wall leakage.et caused surrounding soil salinizated when the process of brine transport. Four soil samples (brine cement, wood chips, mud, mud wells, non-salt polluted soil) from dongyuan mine and 2 salinization of cultivated land soil samples from the liangfeng village of Rong xian were collected in Zigong, Sichuan Province,and a natural saline soil samples collected from Xinjiang Lop Nur as a comparison,so as to study soil physical and chemical indicators, microbial biomass and microbial diversity. Results of this study are as follows:1.The highest PH was 9.62 and the lowest moisture content was 3.03% in the soil of LuoBuBo,that was significant difference with other soil (P<0.05). the highest soil organic matter content was 14.39g/kg in liangfeng village and significantly higher than other samples; the lowest soil organic matter content was 3.55g/kg; and was significant different from other soil (P<0.05). The total nitrogen, efficient and effective potassium content is the highest nitrogen respectively,0.065 g/kg,11.47 mg/kg and 987.33 mg/kg in Eastern Well mine soil that contains a lot of sawdust;.meanwhile,the highest total potassium of liangfeng village soil was 64.22 g/kg,and significant different with other soil samples.2. The influence of salinization stressing on different soil enzyme activity, the content of urease and catalase were 2.57 (NH3-N mg·g-1.24h-1,37℃) and 0.078(0.1mol·L-l KMnO4 ml·g-1,20min-1) respectiveiy in the dongyuan mine, and significantly different with other soil samples (P<0.05). alkaline phosphatase was 1.38 (Phenol mg·g-1±.24h-1,37℃) in dongyuan,which was Significantly higher than other soil (P<0.05).Soil urease and catalase were sensitive to salinization and were Sensitive biology index which can reflect the soil salinization pollution; while inhibitory effect of Alkaline phosphatase subjected was no significantly different.3. Soil salinization and microorganism quantity and microbial biomass carbon were not strictly negative.the quantity of microbial biomass carbon, fungi, actinomyces, nitrogen-fixing bacteria in dongyuan were highest and significantly different from other soils.the content of bacteria was 8.73×105cfu·g-1 in liangfeng village, and significantly higher than other samples (P<0.05)4. Degree of soil salinization, microbial diversity and abundance were highest on land A in liangfeng village, while bacteria and nitrogen-fixing bacteria were on land B. degree of soil salinization was negatively correlative with microbial diversity and abundance, and positively correlative with soil nutrient. No matter salinizated soil or not salinizated soil, or different soil nutrient among salinizated soil, actinobacteria community similarity coefficient was lowest, which indicated that actinomyces was more sensitive to salinization than bacteria, fungi and nitrogen-fixing bacteria. However, the community similarity of fungi was highest, that stated fungi had a good soil salinization patience.5. Total nitrogen, available nitrogen, available K, microbial biomass carbon, urease, catalase and quantity of all kinds of cultivate microbe were significantly higher than other soil samples in the salinizated soil had a lot of sawdust.
Keywords/Search Tags:microbial biomass, soil properties, microbial diversity, DGGE, salt mine, Soil salinization
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