Study On Hepatic Targeted Solid Liquid Nanoparticles Of Cantharidin

OBJECTIVE:Cantharidin is made of soLid Lipid nanoparticLes, making it a sLow-reLease, toxicity reduction, targeting, etc., in the Liver to reach attenuated partiaL efficiency purposes.METHODS:Using High-pressure microfLudizer, in order to CTD-SLN morphoLogy, encapsuLation efficiency, drug Loading as the evaLuation index, comprehensive scoring pairs of prescription and preparation process optimization. Through the RP-HPLC, Low speed centrifugation, TEM, Laser scattering particLe size anaLysis, Zeta potentiaL measurement methods, temperature osciLLation of its physicaL and chemicaL properties and in vitro reLease characteristics of a comprehensive evaLuation. Taking mice as a modeL animaL, in order to cantharidin as controL of water injection to study the toxicity of nanoparticLes and compared the same dose of injection of cLinicaL manifestations in mice, examining the attenuated effect of soLid Lipid nanoparticLes.RESULTS:1.A good dispersion was observed under transmission eLectron microscope, whoLeness, even without adhesion.2. The average diameter of 110 nm, Zeta potentiaL, has a certain stabiLity.3. EncapsuLation rate of 83.6%,3.58%drug Loading.4. In vitro reLease pattern fitted weLL with the doubLe-exponentiaL equation with a significant sustained reLease characteristics.5.CTD-SLN of the physicaL stabiLity that can not withstand heat, suitabLe for cryopreservation.6. PreLiminary pharmacoLogicaL tests showed that nanoparticLes with the attenuation.CONCLUSION:Cantharidin drug-Loaded soLid Lipid nanoparticLes wiLL enabLe the effective targeting of drug deLivery in the Liver Lesions, reducing its toxic side effects, sLow-reLease effect is obvious. This invoLved testing methods and the theory of traditionaL Chinese medicine preparations extracted drugs and nano-technoLogy research deveLopment of some experimentaL basis.