| ObjectiveGastrointestinal ischemia-reperfusion is a basic pathological change for gastrointestinal ulceration, bleeding and dysfunction after the gastrointestinal surgery, systemic inflammatory response and so on. The expression and application of p38 MAPK in gastrointestinal ischemia-reperfusion has little reported home and abroad. This study uses immunohistochemistry methods to detect the expression of p38MAPK and caspase-3 factors in different time after reperfusion, integrating the gastric mucosal damage index and the pathological change with the corresponding time, to export the expression law of p38MAPK and caspase-3 factor. Moreover, this text will discuss their relationship with gastric mucosal injury degree and analysis the expression pertinence between p38MAPK and caspase-3 factors by SPSS 13.0 software.Materials and Methods30 healthy adult SD rats, male and female in half, weight 220±10g, were randomly divided into six groups, sham-operated group (the same surgical procedure without clamping the celiac artery), GI-R groups (reperfusion 0.5h,1h,3h,6h,24h, respectively, after 30min of ischemia).10% chloral hydrate 0.3ml/100g inject into abdominal cavity for anaesthesia. After successful anaesthesia, cut open the abdominal cavity, the celiac artery and its adjacent tissues were carefully isolated. The celiac artery was clamped with a small non-traumatic vascular clamp for 30 min to induce ischemia and then released to allow reperfusion. After reperfusion, the rats were sacrificed to remove the stomach immediately. And then,5 pieces of tissue of 1.0mm*2.0 mm at the greater curvature from the gastric mucosa were immersed in 2.5%glutaraldehyde solution at 4℃for transmission microscopy. The paraffin tissue slices (5μm) were pasted discontinuously to glass pretreated with poly-lysine. The part of paraffin tissue slices was stained with hematoxylin-eosin. After that, use immunohistochemical assay to detect the expression of p38 and caspase-3 of this rats. Compared with the sham group, the GI-R groups were counted in 5 random hign-power (*40) or lower-power (*10) fields. Take IPP map analysis software to detect the IOD and area value, calculate the mean density value with each group, and express as mean±D. At last, use spss 13.0 software to statistic the data, selecta=0.05 as a standard.Result and conclusionThe gastric mucosal express p38MAPK and caspase-3 factor in false operation group much less than operation group. After gastric ischemia-reperfusion (GI-R) caspase-3 expression increase rapidly, and it reach first peak in 0.5h, and then decline, to 3h it go to the bottom, and then increase slowly, to 24h it reach the maximum value. As to p38MAPK expression, lh it reach its peak, and then it decline, but its decline trend has no any statistic meaning, because it still stay in high level. Meanwhile, gastric mucosal damage index increasing when start with GI-R. and it reach its peak in 1h, with the time going on, the injury of gastric mucosal start to repair, so the damage index decline in time, going to bottom at 3h, but then it climbs slowly within 3h to 6h, that its to say, the injury aggravation,6h to 24h that is no significant change.As we seen from above result, the expressions of p38MAPK and caspase-3 factor have association with the gastric mucosal damage index in the progress of gastric ischemia-reperfusion injury. At first they show the positive correction with each other. Gastric ischemia-reperfusion lesion and repair are caused by multiple factors. P38MAPK regulate and control the inflammatory response and cells apoptotic, promoting gastric ischemia-reperfusion injury form and development. |
PDF Full Text Request