The Research About Feridex Labeled Mscs Imaging Tracer In 3.0t Magnetic Resonance In Vitro

[objective] To study the superparamagnetic iron oxide particles (superparamagnetic iron oxide particles, SPIO) labeled SD in vitro rat bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) the feasibility of its SD rat bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) biological activity. Of superparamagnetic iron oxide particles (superparamagnetic iron oxide particles, SPIO) in vitro marker of SD rat bone marrow mesenchymal stem cell (mesenchymal stem cells, MSCs) in the cell under 3.0T Magnetic Resonance Imaging Tracer feasibility..[materials and methods]1. Isolated MSCs from SD rat bone marrow, culture and identification of MSCs.2.1n vitro combined with different concentrations of poly-lysine tag SPIO, unlabeled cells as control. Prussian blue staining and electron microscopy the cells were iron particles.3.Trypan blue staining to detect cell survival.4.Magnetic marker MSCs into adipogenic, osteogenic induction medium after culture were identified.5.Application 3.0T Magnetic Resonance h2d1₁54 sequences and tseBR2dl₁6 sequences labeled cells on magnetic imaging.[Conclusions]1. Prussian blue staining and electron microscopy showed dense cytoplasm containing iron particles2.Trypan blue staining showed that activity of magnetic markers on MSCs was no significant difference (P> 0.05)3.labeled cells can be normal as fat, bone formation cell differentiation.4.h2d1₁54 sequence and tseBR2d1₁6 sequence tips and unlabeled cells in signal strength (SI) compared to,1×106 (labeled cells),5×105 (labeled cells) SI were significantly decreased (P<0.05), which h2dl₁54 sequence signal strength decay rate (ΔSI) were significantly higher than unlabeled group (P<0.05). In the two sequences 1 x 106 (labeled cells)ΔSI higher than 5×105 (labeled cells)ΔSI, but no significant difference (P> 0.05).[Conclusion]Combined SPIO polylysine successful tag MSCs, magnetic markers and potential differentiation of cells had no effect. Magnetic labeled cells to generate the MR signal changes characteristic of the low. Application 3.0TMR imaging tracer labeled cells is feasible to h2d1₁54 most sensitive imaging sequence.