Regulatory Effect Of Tripterygium Wilfordii Polyglycoside On Expression Of Egfr Family In Cia Rats

Objective: To study the regulatory effect of Tripterygium Wilfordii Polyglycoside (TWP) on the expression of EGFR and ERBB-2 of collagen induced arthritis rats.Methods: After the model of CIA rats was developed, rats were randomly devided into model group, the TWP group and the MTX group. Tissues were taken from the rats after 4 weeks treatment. The articular volume was measured and the synovium was examined with H&E staining. In addition, the expression of EGFR and ErbB-2 in synovium and articular cartilage were tested by immunohistochemical strain and real time PCR.Results: The protein and mRNA expression of EGFR and ErbB-2 in the synovium and articular cartilage of the model group were significant higher than the control group (p<0.01). Having been treated with TWP and MTX, the protein and mRNA expression of EGFR and ErbB-2 decreased markedly (p<0.01), but they were higher than the control group (p<0.05). Difference between the two treatment groups was not significant(p>0.05).Conclusion: EGFR and ErbB-2, members of EGFR family, may play an important role in the process of arthritis development. The molecular mechanism that TWP treat synovitis and bone destruction of RA is correlating with decreasing expression of EGFR and ErbB-2. Objective: To study the regulatory effect of Tripterygium Wilfordii Polyglycoside (TWP) on the expression of PARP-1 of collagen induced arthritis rats.Methods: After the model of CIA rats was developed, the expression of PARP-1 in synovium and articular cartilage were tested by immunohistochemical strain and real time PCR. Results: The protein and mRNA expression of PARP-1 in the synovium and articular cartilage of the model group were significant higher than the control group (p<0.01). Having been treated with TWP and MTX, the protein and mRNA expression of PARP-1 decreased markedly (p<0.01).Conclusion: PARP-1 may play an important role in the process of arthritis development. The therapeutic effect of TWP may be related to decreasing expression of PARP-1.