Effect Of Proliferation Inhibition And Apoptosis Mechanism On Multiple Myeloma Cells Induced By Asiatic Acid

AIM:To explore the effect of suppression growth ,induction apoptosis ,distribution of cells cycle induced by asiatic acid on human multiple myeloma cells RPMI8226 ,and its antitumor mechanism.METHODS:MTT assay was used to assess the effect of asiatic acid on cells proliferation;Hoechest33258 staining was studied to evaluate the morphological changes in RPMI8226 cells induced by asiatic acid;flow cytometry was used to analyze the induction apoptosis and cell cycle arrest of asiatic acid;RT-PCR was applied to detect the changes of survivin ,bcl-2 mRNA expression levels.RESULTS:Asiatic acid significantly inhibit cell proliferation of RPMI8226 inμmol/L grade level,in a time and dose-dependent manner.The IC50 of RPMI8226 cells were (42.25±4.57)μmol/L,(24.88±3.51)μmol/L,(19.83±2.88)μmol/L for 24 hours,48 hours,72 hours .Asiatic acid can induce apoptosis ,and cell showed typical apoptotic features.Asiatic acid induced apoptosis in a dose-dependent manner.Cells treated with asiatic acid 25～40μmol/L were blocked in the G2 phase.The expression of survivin and bcl-2 mRNA were decreased in RPMI8226 cells,and the extent of apoptosis was a negative correlation with the dose of asiatic acid.CONCLUSIONS:Asiatic acid can suppress the proliferation by induceing apoptosis and regulating cell cycle process.The mechanism of induction apoptosis was associated with up-regulation of survivin and bcl-2 transcription level.