Expression Of Copper-transporting P-type Adenosine Triphosphatase (atp7b) Correlates With Cisplatin-resistance In Human Lung Adenocarcinoma Cell Line A549

Background and objective Cisplatin is the basic chemotherapy drug of lung carcinoma, and cisplatin-resistance mostly leads to the failing of chemotherapy in the patients. The aim of this study is to investigate the relationship between the expression of copper-transporting P-type adenosine triphosphatase (ATP7B) and cisplatin-resistance in different cisplatin-resistant A549 cells[1].Methods Three differently cisplatin-resistant A549 sublines(A549/DDP0.5,A549/DDP1.0,A549/DDP2.0) were established from their parental human lung adenocarcinoma cell line A549 which was sensitive to cisplatin, by gradually increasing concentration of cisplatin. The resistance indexes of all the sublines were checked by MTT method. The levels of ATP7B mRNA and protein were measured by RT-PCR and Westen blot respectively in all the cell lines[1].Results The resistance indexes of the three cisplatin-resistant sublines were 1.7, 3.2 and 5.2 respectively(P<0.001), and the levels of ATP7B mRNA were 1.6, 4.9 and 10.1 folds compared to the parental A549 cells(P<0.001). Meanwhile, expression of ATP7B protein was enhanced in the three cisplatin-resistant sulines corresponding to their cisplatin-resistance[1]. conclusion Expression of ATP7B correlates with cisplatin-resistance in A549 cells, and maybe ATP7B contributes to acquisition of cisplatin-resistance[1].