The Research Of Apoptosis Introduction And Proliferation Inhibition Of Hep-2 Cells By Water Extract Of Scutellaria Barbata D·don

Objective :This study investigated the apoptosis introduction and proliferation inhibition of Hep-2 cells by water extract of Scutellaria barbata D·Don.Material and Methods:The water-soluble components of Scutellaria barbata D·Don was extracted. The proliferation inhibition of Hep-2 cells was determined by inhibition ratio using CCK-8 after exposure to different concentrations of water extract of Scutellaria barbata D·Don (0.1mg/mL,0.2mg/mL,0.5 mg/mL,1 mg/mL,2 mg/mL,3 mg/mL) for 24h,48h and 72h. The apoptosis of Hep-2 cells labeled Annexin-V-FITC/PI were analysis by flow cytometry, and the nucleus DAPI staining were observed after treated with water extract of Scutellaria barbata D·Don (0.5 mg/mL,1 mg/mL,2 mg/mL) for 48h.Results: Water extract of Scutellaria barbata could inhibit the proliferation of Hep-2 cells effectively. Flow cytometry analysis showed that water extract of Scutellaria barbata could induces apoptosis in Hep-2 cells, and the early and late apoptosis were increased in a time and concentrations-dependent manner after treatment of Hep-2 cells with water extract of Scutellaria barbata (P<0.05, respectively). It showed that nuclear pyknosis, nuclear fragmentation, chromatin condensation and other morphological changes typical of apoptosis-like nucleus in Hep-2 cells after treatment with water extract of Scutellaria barbata under fluorescence microscope.Conclusion Water extract of Scutellaria barbata can inhibit the cell proliferation by inducing apoptosis in Hep-2 cells.