Selection And Initial Characterization Of Humanized Anti-paraquat Scfv

Objective To screen humanized anti-paraquat scFv from large human phage antibody library ,and its specific binding properties with paraquat artifical antigen were identified.Methods In the process of "absorption - elution - amplification" , using solid-phase ELISA-based antigen screening method, that artificial antigen paraquat (PQ-h-BSA) as antigen,choosing by their specific binding properties of the initial identification in ELISA, to screen humanized anti-paraquat scFv from large human phage antibody library .Results Two strains with paraquat artificial antigen-specific combination of positive clones were got in 5 rounds of screening. In initially identified by ELISA, anti-paraquat scFv of screeninig reacted strongly with paraquat artifical antigen , but no reaction or weak with other antigens.Conclusion It is feasible to obtain humanized anti-paraquat scFv by phage display technique,which provides a more promising source of small molecule human antibodies for clinical treatment of paraquat poisoning study.