Dopamine D1 Receptor And Nmda Receptor Involved In Regulation Of The Expression Of △fosb In The Striatum Of Rats With Levodopa Induced Dyskinesias

Objective To investigate the effect of dopamine D1 ,dopamine D2 receptor and glutamate NMDA receptor on the behavior and expression of△FosB protein in the rat with Levodopa-induced Dyskinesia.Methods Unilaterally lesioned rat model of Parkinson's disease was established by using 6-hydroxydopamine, and the rat model was treated with levodopa and benserazide daily for 28 days. All rats were divided into seven groups:normal control group, PD group, LID group, SCH23390 group, MK-801 group, raclopride group and non-LID group. The behavioral changes were observed and the score of abnormal involuntary movement(AIM score) were assessed by using the rat AIM scale. The expression of△FosB protein in striatum were measured and compared in each group using immuno-histochemistry assay and western blotting.Results Both Dopamine D1 receptor antagonist SCH23390 and NMDA receptor antagonist MK-801 attenuated abnormal behavior of LID rats, while dopamine D2 receptor antagonist raclopride had no significant effect on abnormal involuntary movement;△FosB in the lesioned striatum of LID rats was expressed more than that of PD rats and non-LID rats, and the upregulation of△FosB in the lesioned striatum of LID rats could be reduced by SCH23390 and MK-801 but not by raclopride. There was no significant difference in expression of△FosB protein in intact striatum among seven groups.Conclusion Both Dopamine D1 receptor and NMDA receptor were involved in development in the LID rats by regulating the expression of△FosB in the lesioned striatum.