The Research Of An Animal Model On Alcohol-induced Osteonecrosis In Short-term

Background:With the global consumption of alcoholic beverages increasing, alcohol-induced osteonecrosis of the femoral head patients also were increasing. Alcohol-induced osteonecrosis of the femoral head was particularly prevalent in middle-aged and young male long-term alcohol abuse. The disease, which has high disability and severely affected patients with the ability to work and quality of life, has aroused great attention from clinicians. Going with the deep research of the prevention and treatment of the disease, it is particularly important to establishment an ideal animal model.Objective:To study the effect of alcohol to the femoral head of rabbit in sensitive condition.Methods:48 male rabbits were randomly divided into four groups. In Group A, animals were injected 10 ml/kg of horse serum intravenously twice with the interval of three weeks, then poured into stomach with normal saline in 10 ml/(kg·d) 2 weeks later. In Group B, animals were administered the strong wine (The volume fraction is 46% alcohol) in 10 ml/ (kg·d) on the synchronization, as same time, to Group A poured normal saline. In Group C, animals were injected 10 ml/kg horse serum intravenously twice with the interval of three weeks, then administered the wine (The volume fraction is 46% alcohol) in 10 ml/(kg·d) two weeks later. In Group D, animals were poured the normal saline in 10 ml/ (kg·d) as control. The animals in the experiment were sacrificed in batches four and eight weeks after pouring ethanol into stomach. The pathological changes of the femoral head were studied.Results:In histopathological examination after 8 weeks in Group C, the pathology change mainly concentrates under the rabbit femoral head cartilage . The hematopoietic tissue in the femoral head marrow decreased and the fatty tissue increased, fatty cells in the head increased and enlarged were observed. The trabeculace became thinner and sparse, and its area fraction reduced. The percentage of empty osteocyte lacunae was increased and were statistically significant differenves compared with the results in group B,C and D ( at 4 weeks P<0.05) and (at 8 weeks P<0.01) .There were no obviously pathological changes in the other 3 groups.Conclusion:The shot-term excessive intake of the wine can induce the osteonecrosis of the femoral head under sensitive condition.