The Immunity Response In Mice Of The Nucleic Acid Vaccine Gapdh In Brugia Malayi And The Gene Cloning Of Bm-cpi

Filariasis as a epidemic is a serious hazard to human health. The drug-resistance of filaria and mosquito has been increasing owing to making use of chemical medicine. The need for vaccines to relieve and wipe out the resurgence of filariasis is urgen. Developing an effective filariasis vaccine is still an important task which scientists face for. Compared with protein vaccine, nucleic acid vaccine which is developed recent years has many advantages. Existing datas indicate that glyceraldehyde-3-phosphate dehydrogenase(GAPDH)in parasitic worms and protozoa can produce protective immune response in laboratory animals and that is an important vaccine candidate antigen. This study constructed the nucleic acid vaccine gapdh of Brugia malayi, which is used to immune BALB/c mice with the immune adjuvant CpG. Then, we detected the immune response using RT-PCR, MTT, ELISA. The results show that the nucleic acid vaccine pcDNA3.1/Bm-gapdh may be a potential nucleic acid vaccine candidate to resist filariasis. Preparation for the next, we also constructed recombinant plasmid of cysteine protease inhibitor(CPI)in Brugia malayi (pGEM-T/Bm-cpi), which was sent to the gene company to sequencing. According to nucleotide sequence, their amino acid sequences were inferred. Then the B cell epitope of Bm-cpi were predicted by various corresponding software.