The Effect Of Pha Biomaterial Scaffolds On The Osteogenic Differentiation Of Umbilical Cord Mesenchymal Stem Cells

Polyhydroxyalkanoates (PHAs) have been shown to be good biomaterials in tissue engineering, and mesenchymal stem cells (MSCs) has widely source, Easily isolated, no immunity, good skills of division and proliferation and can differentiate to nerve cell, adipose cell, skeletogenous cells, and so on. In this study, human umbilical cord mesenchymal stem cells (UC-MSCs) were transplanted into the PHA biomaterial scaffolds and induced to differentiate into osteoblasts. CCK-8 analysis was performed to detect the cell proliferation, mechanical properties were tested for each biomaterial, von Kossa and ALP staining and RT-PCR were used to detect the osteogenic differentiation of UC-MSCs. P3HB4HB, PHBHHx and PHBVHHx scaffolds were compared for their application in bone tissue engineering.In this study, UC-MSCs were seeded into PHBHHx, P3HB4HB and PHBVHHx scaffolds and cultured for different days. Compared with cells cultured in 24-well cell culture plates, CCK-8 assay showed that the three PHA biomaterial scaffolds promoted more cell proliferation and PHBVHHx scaffold showed the highest cell proliferation. Among the three PHA biomaterials, physical analysis showed that P3HB4HB was hardest and most brittle, PHBVHHx was the softest and most flexible, and PHBHHx was between the two other biomaterials. Von Kossa and ALP staining detected the black calcium deposit and the red alkaline phosphatase expression respectively, indicating that UC-MSCs could be differentiated into osteoblasts in P3HB4HB, PHBHHx and PHBVHHx scaffolds. RT-PCR analysis also proved the ostrogenic differentiation of UC-MSCs in the scaffolds. In conclusion, P3HB4HB, PHBHHx and PHBVHHx biomaterials has good biocompatibility for UC-MSCs, can effectively stimulate UC-MSC proliferation and support the osteogenic differentiation of UC-MSCs. Among the three biomateials tested, PHBVHHx promoted the highest UC-MSC proliferation. Among the three biomateials tested, P3HB4HB was the hardest and was suitable for the repair of bone and cartilage damage; PHBVHHx was the softest and was suitable for the repair of soft tissue damage.