Bone Marrow Mesenchymal Stem Crisp Treatment Of The Role Of The Sleeve Of A Robe Bloody Brain Temple Injury Recovery Period

Part 1 Effects of bone marrow mesenchymal stem cells transplantation on neurological function and the expressions of angiopoietin 1,2 and their receptor at recovery stage of cerebral ischemiaObjectives:To explore the effects of bone marrow mesenchymal stem cells (BMSCs) transplantation on neurological function and the expressions of angiopoietin 1,2 and their receptor tyrosine kinase with immunoglobulin-like and epidermal growth factor homology domains-2(Tie-2) at recovery stage of cerebral ischemia.Methods:Middle cerebral artery occlusion (MCAO) model of cerebral ischemia SD rats was made with thread embolism method, modified adhesive removal test (MST) was carried for the assessment of neurological function of the rats. At 21d after cerebral ischemia, BMSCs transplanted group was administrated with BMSCs through tail vein, while control group was administrated with the same volume of PBS. At 28d, 35d and 42d after cerebral ischemia (7,14 and 21 days after BMSCs administration), RT-PCR and Western blotting were performed for the mRNA and protein detection of angiopoietins 1,2 (Ang-1,2) and their receptor (Tie-2) in the cerebral ischemic boundary tissues, and then compare with the sham-operated group.Results:The MST of BMSCs transplanted group was significantly higher than that of control group in subgroups at each time point (P<0.01). In subgroups at each time point, the mRNA and protein expressions of Ang-1 and Tie-2 in the cerebral ischemic boundary tissues in the BMSCs transplanted group and the control group were significantly increased than those of the sham-operated group (P<0.01). In subgroups 28d and 35d after cerebral ischemia, the mRNA and protein of Ang-1 and Tie-2 expressions in the cerebral ischemic boundary tissues were markedly elevated in the BMSCs transplanted group than those in the control group(P<0.01). However, no significant difference of those parameters was found after the cerebral ischemia 42d between two groups. In three groups at each time point, the mRNA and protein of Ang-2 expressions in the cerebral ischemic boundary tissues were not of significant changes.Conclusions:Administration of BMSCs at the recovery stage of cerebral ischemia may improve the neurological functions of model rats as well as increase the expressions of Ang-1 and Tie-2 in the cerebral ischemic boundary tissues, but not for the those of Ang-2. Part 2 Effects of bone marrow mesenchymal stem cells transplant on the phosphorylation of cAMP response element binding protein and its target gene brain derived neurotrophic factor at recovery stage of cerebral ischemiaObjectives:To explore the effects of bone marrow mesenchymal stem cells (BMSCs) transplantation on the phosphorylation of cAMP response element binding protein and its target gene brain derived neurotrophic factor(BDNF) at recovery stage of cerebral ischemia.Methods:MCAO model of cerebral ischemia SD rats were made with thread embolism method. At 21d after cerebral ischemia, BMSCs transplanted group was administrated with BMSCs through tail vein, while control group was with the same volume of PBS. At 28d, 35d and 42d after cerebral ischemia (7,14 and 21 days after BMSCs administration), western blotting were performed for the protein detection of CREB and pCREB, and RT-PCR were performed for the mRNA detection of BDNF in the cerebral ischemic boundary tissues, and then compare with the sham-operated group.Results:In subgroups 28d and 35d after cerebral ischemia, the protein of pCREB expressions in the cerebral ischemic boundary tissues were significantly elevated in the BMSCs transplanted group than that of control group(P<0.01). However, no significant chage was found after the cerebral ischemia 42d between two groups. The protein expressions of pCREB in control group didn't change significantly Compared with that of sham-operated group at each time point. In three groups at each time point, the protein of CREB expressions in the cerebral ischemic boundary tissues were not of significant change. In subgroups after cerebral ischemia 28d and 35d, the mRNA of BDNF expressions in the cerebral ischemic boundary tissues were significantly elevated in the BMSCs transplanted group than that of control group(P<0.01). However, no significant change was found after the cerebral ischemia 42d between two groups. There was no statistical change between control group and sham-operated group for BDNF mRNA at each time point, neither.Conclusions:Administration of BMSCs at the recovery stage of cerebral ischemia may elevate the phosphorylation of CREB and the mRNA expressions of its target gene BDNF in the cerebral ischemic boundary tissues.