High-frequency Pulsed Electromagnetic Fields On Bone Marrow Mesenchymal Stem Cell Proliferation And Differentiation,

BACKGROUND The researches about low-frequency pulsed electromagnetic fields (PEMFs) interfering with bone marrow mesenchymal stem cells (BMSCs) proliferation and differentiation are many, but there is no report concerning high-frequency (380MHz) PEMFs.OBJECTIVE To explore whether high-frequency (380MHz) PEMFs can promote proliferation and osteogenic differentiation of BMSCs.METHODS BMSCs were isolated from Sprague-Dawley rats. The third passage of BMSCs were obtained and divided into four groups: osteogenic induction group, osteogenic induction+PEMF irradiation group, PEMF irradiation group and blank control group. Changes in morphology, number and amount of total protein were observed during BMSC culture in each group.RESULTS The number of BMSC bodies was increased, but the differentiation ability was weak following PEMF irradiation. The speed of cell proliferation and content of total protein were lower in the PEMF irradiation group compared with blank control group (P< 0.05). However, cell apoptotic rate was greater in the PEMF irradiation group compared with blank control group (P< 0.05).CONCLUSION Results indicated that promoting effects of high-frequency PEMFs on osteogenic differentiation of BMSCs are not obvious. High-frequency PEMFs can suppress BMSC proliferation and contribute to BMSC apoptosis. BACKGROUND The researches about low-frequency pulsed electromagnetic fields (PEMFs) interfering with bone marrow mesenchymal stem cells (BMSCs) proliferation and differentiation are so many, but by surface Raman spectroscopy analysis of electromagnetic field irradiated by the Raman spectra of single stem cells rarely reported.OBJECTIVE using Raman spectrometric to observe and analysis biology impact of BMSCs after the radiation by high-frequency (380MHz) PEMFs.METHODS BMSCs were isolated from Sprague-Dawley rats, passage 3 cells were inoculated into six-well plates and divided into 2 groups:D is PEMF irradiation group and C is blank control group. Collecting Raman spectra from survival of the state of bone marrow mesenchymal stem cells after cultured for 7 days. Analyze the data of two sets for comparison. RESULTS①The cuve of D is similar to C;②The peaks of 380MHz PEMF irrigation group were lower than control group,the peaks were especially lower likes 524,650,764,1302,1367,1561cm-1 and so on;③It's no different even though the peaks (966,1206 cm-1) of Hydroxyapatite and hydoxyprolin were found both C and D.CONCLUSION①Laser optical tweezers Raman spectroscopy can be applied to a single stem cell at the molecular level of biochemical changes in the measurement and research;②It's useful that Raman spectroscopy analysis of the impact of electromagnetic fields on cells is a feasible approach.