Fusarium Moniliforme Balb / C Mice Pathogenicity

Object:To establish successfully an experimental model of disseminated Fusarium moniliforme infection and to investigate the pathogenicity of Fusarium moniliforme in BALB/c mice. To explore the pathogenic factors and the visceral involvement in murine disseminated Fusarium moniliforme infection in normal and immunosuppressed mouse.Methods:60 mice were separated into 5 groups randomly and medially.Two suspension dosages(1×106cfu/animal,5×107cfu/animal)were tested.Two immunocompromised groups were intraperitoneally inoculated with two suspension dosages separately as well as the other two normal groups. Control group were intraperitoneally inoculated with the same dosage were tested. Systemic symptom (appetite, mental condition,weight,hair,live time) were examined each day.On 28d, mice were killed and Macroscopic observtion, fungal culture and tissue pathology were examined in heart,liver, lungs, spleen,kidney and brain of dead or killed mouse. Fungal culture and tissue pathology(HE dyeing and PAS dyeing) of visceral organs were done. Fungal culture of mouse blood were examined.Results:1.The survival state and time of mice:The activity and food intake of immunocompetent mouse had no obvious change after inoculated intraperitoneal with Fusarium moniliforme. The immunsuppressed mice reduced food-intake and activity in different degree, C and D group weight loss, hair fall against the dull. The weight of immunsuppressed mice descent and their hairs were dark. The activity and food intake of D group significantly decreased compared with C group after inoculated intraperitoneal with Fusarium moniliforme. Mortality of mice in each group were A, B, E group were 0%(0/12), C group was 16.7%(2/12), D group was 100%(12/12).2.Fungal culture:Normal group (A, B group) and control group E group organ fungal cultures were negative, blood cultures also were negative. Immunosuppression group (C, D group) were positive fungal culture.3.Histopthologic examination:HE dyeing showed inflammatory cell infiltration, vascular dilation, organization degeneration and necrosis.PAS dyeing displayed mauve hypha in spleen,heart and liver.4.The morbidity in the immunosuppressed mice was 100%.Conclusion:1.Fusarium moniliforme is ane opportunistic pathogen which can lead to the infection of the viscera in immunosuppressed mice. Common pathological features of viscera infection is inflammatory cell infiltration, vascular dilation, organization degeneration and necrosis.2.Fusarium moniliforme can cause immunosuppressed murine systemic infecti- on through intraperitoeal.3. Fusarium may invade the spleen, liver, heart, kidney, lung and brain. Spleen may be the most vulnerable to invasion of viscera.