Konjac Glucomannan / Type Ii Collagen / Chitosan Preparation And Performance Of The Three-phase Composite Materials Research

Skin is the body's largest organ.The skin wounds, such as burns, varicose veins, dermatosis, cancer skin and canker repairs difficultly because of the lack autologous transplantation of skin graft. Although there is autologous skin Transplantation, heterologous skin transplantation and many other methods.But they can not adapt all the needs and exhibit limitations.Therefore there is a great demand to study a biocompatibily. Biodegradable tissue engineering material which can be adapted for repair the organ such as skin.For the present study used tissue-engineered skin polymer film of moisture absorption and water permeability of the bad question, is hereby made to the preparation of konjac glucomannan(KGM)as the base of the blend membranes.KGM is a type of natural macromolecule material with fine degradation and film forming characteristic,the film of KGM has good mechanical property.Type ofⅡcollagen protein (CollagenⅡ,COLⅡ),which has good biological characteristics, is the main structural protein in bodies of human beings and vertebrates.But COLⅡis mechanically weak and easy to biodegrade. Chitosan (CS) is a kind of natural polymer which has good biocompatibility and biodegradability.Three stand-alone applications with different defects, it is difficult to meet the tissue engineering of biological material performance requirements.So this paper put forward on this basis to KGM,COLⅡ,CS as the main raw materials to prepare of three-phase composite materials. Therefore make up those who use less than their own.KGM/COLⅡ/CS blend films were prepared by solvent-casting method linked orthogonal design L9(34).CS was dissolved with 36% acetic acid.COLⅡwas mixed in the glycerol solution.The two liquids were blended to form a sol.The sol were poured into the disks.The disks were putted into the decompresssion condition to dry into films.The acetic acidin the films were neutralization via sodium hydroxide solution.KGM/COLⅡ/CS composite membrane phase to the mechanical properties of blend film elongation at break,tensile strength,surface properties of membrane thickness and water absorption as part of an integrated assessment.The best raw materials with than:KGM for 1g, COLⅡfor 0.6g, CS for 0.5g, glycerol 0.2ml,36% acetic acid 0.5ml, distilled water 100ml to preparing the membrane.Best KGM/COLⅡ/CS membrane three tensile strength for 86.29MPa, elongation at break of 8.8%, thickness to 0.124 mm, water absorption up to 700 percent, so the skin can reduce the blend film plot fluid and prevent multiplication of bacteria. KGM/COLⅡ/CS blends skin membrane has good water vapor through the coefficient reached 2.49g·mil/day·m2·Pa. Through XRD analysis, CS,COLⅡ,KGM occurred between hydrogen bonding.The CS apart off the characteristics group-acetyl.So the film was amorphous state.By FT-IR analysis of the availability of KGM/COLⅡ/CS blend membranes,CS,COLII,KGM'structure didn't be changed.But there were a strong hydrogen bond between the role.KGM was in substantial removal of acetyl and hydroxy also changed.CS was also a higher degree of deacetylation.By KGM/COLⅡ/CS blend membranes of skin transmittance rate (<50%) and dry micro-morphology, wet SEM electron microscopy analysis can reflect the relationship between the good compatibility. KGM/COLⅡ/CS blend films in vitro analysis show that the materials degradated about six weeks. The human skin tissue metabolism time about 4 or 5 weeks. In vitro bone mesenchymal stem cells (BMSCs)and human periodontal fibroblasts cells(PFCs) proved the films with good affinity and compatibility.The PFCs of the muscle cell responsed that the material can be used as the skin can promote the use of skin scaffords and periodontal.