Different Concentrations Of Oxidized Low-density Lipoprotein On Murine Macrophage Ppar¦Ä Expression

Objectives Foam cell is a key factor to atherosclerosis. To observe the PPARδmRNA and the acceptor expression at different concentrations of ox-LDL induces the mouse macrophage cell Raw264.7 into foam cell,so as to illustrate the function of PPARδin the foam procession..Methods The mouse RAW264.7 cells was induced into foam cells by incubation with oxidized low density lipoprotein(ox-LDL) at the respectively concertrationg of 0,20,40,80 mg/L. The cellular lipid accumulation was examined by oil red O stainin at 24h.The expression of PPARδimmune body was observed by the method of immunity fluorescence. The RNA was extracted at 6h, and then the expression of PPARδmRNA was detected by RT-PCR.Results The oil red showed that the control group (the physiological saline group) wasn't dyed nearly, ox-LDL group showed that the massive cell cytoplasm was dyed garnet by oil red O, the cell nucleus was dyesd purple.With the increment of the concentrations of ox-LDL, the cytoplasm colour was more deeper ,the content of intracellular lipid in the cells was elevated. Immunity fluorescence showed that with the increment of the concentrations of ox-LDL, PPARδantibody fluorescence intensity became more brighter, PPARδantibody was upregulated .Real-Time-PCR results analysis demonstrated that each group PPARδDNA relative grey level was higher than control group(P<0.05).Single factor variance analysis manifestated that PPARδmRNA is dose dependent by concentrations of ox-LDL (P<0.025). It's have Statistics significance.Conclision PPARδhave the function to regulate the Raw264.7 cell foam procession. With the increment of the concentrations of ox-LDL, the regulation of PPARδto foam procession is increase.Wheather gene level or protein level.Isn't prove that PPARδhave the negative function in Raw264.7 cell foam procession.