| Objective:In this study, we investigated the probability and effectivity of the magnetic nanocomposite composite medium as a novel mediator of magnetic induction hyperthermia (MIH) and chemotherapy for gliomas treatment.To research the nature of good preparation method of magnetic medium by preparation materials. In vivo experiments confirm the safety of magnetic nano-composite medium, as well as the release of chemotherapy combined with hyperthermia and the inhibition of tumor growth and prevent tumor recurrence.Methods:The preparation of magnetic nanoparticles (MNPs) and docetaxel PLGA nano-release microspheres:preparation the magnetic fluid by chemical coprecipitation,coated with silica and modified with a defined concentration of APTES. Hysical and chemical characterization by infrared spectroscopy, Transmission electron microscopy, vibrating sample magnetometer, thermal analysis; Preparation the docetaxel PLGA controlled release microspheres by solvent evaporation method. Physical and chemical characterizati on high performance liquid and scanning electron microscopy.In vivo experiment investigate the hot synergistic effect of docetaxel PLGA controlled release microspheres:Established subcutaneous U251 tumor model in nude mice, Intratumoral injection of magnetic nanoparticles, docetaxel PLGA nano-release microspheres and magnetic nanocomposite composite medium, Magnetic induction thermal chemotherapy in 43℃,20min. After treatment, tumor growth was observed in each group to observe if the treatment can inhibit tumor growth and recurrence and prolong survival. Recorded data were compared.Results1. Preparation the magnetic fluid by chemical coprecipitation, the dispersion is good after coated with silica. TEM showed that the particle surface coated with a layer of film. FTIR showed a characteristic peak of amino groups after modified with APTES. The modified magnetic nanoparticles in alternating magnetic field has a good heating effect, and the temperature increased with the increasing content of nanoparticles and magnetic field strength. All the magnetic nanoparticles are superparamagnetic. TEM showed a good dispersion. Preparation nano-release microspheres by solvent evaporation method. Magnetic induction heating can promote the release of drugs. Magnetic nanocomposite composite medium including magnetic nanoparticles and nano-release microspheres. They linked by charges attract. TEM results showed that the magnetic nanoparticles attached to the microspheres surface.2. U251 gliomas cell were subcutaneously injected in nude mice, who were randomly divided into 6 groups. The group which is intratumoral injection of magnetic nanoparticles, showed that nano-composite medium is not toxiced. Thermothempy and thermochemotherapy groups heated regularly for 20 minutes for 3 times. Both groups were observed the phenomenon of tumor regression, and the control group was statistically significant. The effect of thermochemotherapy group was better than thermothempy group, for the tumor recurrence rate was lower. The effect of chemotherapy group and the nano-release microspheres group was not good, but the tumor growth slowly in nano-release microspheres group.ConclusionThe magnetic nanocomposite composite medium can heating under alternating magnetic field, it's good physics and chemistry characters can meet clinical requirements. Meanwhile, the mediun can realize sustainable release of docetaxel, which combined with magnetic fluid mediate, enhance the effect of anti-tumor. The magnetic nanocomposite composite medium is an effective medium of thermal-chemotherapy and has broad application prospects. |
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