Salt The Licorice Acid And Liquiritin Of Accumulated Its Mechanism Of Action

The environmental conditions and cultivation technique greatly influented the cultivated herbs. It has become a hot topic that how to stabilize and improve the herbs quality. So the researches took Glycyrrhiza uralensis Fisch as material, some aspects were analysed, including the growth, yield, physiology, material components and biosynthetic pathway under varied salt treatment to receal the influence and function mechanism of salt treatment on glycyrrhizic acid and liquiritin accumulation. Thus, the purpose was to stabilize and improve the quality of Glycyrrhiza uralensis Fisch. The main results of the research were stated as follows:1. The growth and yield were inhibited under salt treatment. The growth and yield of 0.6% and 0.9% treatment were significantly decreased in the mid-late stage.2. The contents of glycyrrhizic acid and liquiritin were affected under salt treatment for adapting to adverse circumstances by regulating physiological characteristics. The results were summarized as follows:(1) Salt treatment had a significant influence on the antioxidant enzymes activities. Glycyrrhizae uralensis Fisch could adapt to adverse circumstances by increasing the activities of SOD,POD and CAT under salt treatment. The antioxidant enzymes activities had a positve correlation with the relative content of glycyrrhizic acid and liquiritin.(2) Salt treatment altered photosynthesis characteristics indexes. Under salt treatment, the content of Chlorophyll was decreased, and the plant had a higer value of Chla/Chlb. And Pn, Tr and Gs all decreased along with the increase of salt treatment degree, while Ci in treatment group was lower than control group at the early stage, but higer at the late stage.3. The contents of the material compositions were changed to make change the contents of glycyrrhizic acid and liquiritin under salt treatment. The amount of total sugar was decreased, while the amounts of crude protein, crude fiber, crude fat and ash were increased under treatment condition. At the late treatment stage, the crude fat proportion of 0.9% treatment group was extremely higher than that of control group, while the total sugar proportion was extremely lower. The total sugar content had a negative correlation with relative content of glycyrrhizic acid and liquiritin.4. The content of biosynthetic prosoma of glycyrrhizic acid was changed under salt stress, while the key enzyme expression in synthesis of glycyrrhizic acid was enhanced. The content of squalene was decreased after salt treatment, while the content ofβ-amyrin and theβ-amyrin synthase expression was increased. It was that the content of squalene under 0.9% treatment was the lowest,β-amyrin content and theβ-amyrin synthase expression were the higest. The relative content of glycyrrhizic acid had a positive correlaion withβ-amyrin synthase expression. While the absolute content had a negative correlaion withβ-amyrin content.5. The relative contents of glycyrrhizic acid and liquiritin were improved, and 0.9% treatment group was significantly higher than control group. While the absolute contents of glycyrrhizic acid and liquiritin in treatment group were lower than in control group, and content in the 0.9% treatment group was minimum. The relative contents of glycyrrhizic acid and liquiritin had a negative correlation with various growth indexes, but the absolute contents had a positive correlation with growth indexes.6. The possible mechanism of salt treatment on glycyrrhizic acid and liquiritin was as following:The increase of glycyrrhizic acid and liquiritin relative content after salt treatment inhibited primary metabolism and developed secondary metabolism. The former mainly were in inhibiting the growth of shoot dry weight, improving body antioxidant enzyme activities, decreasing net photo synthetic rate, thereby reducing the total sugar content of primary metabolites; the latter was about increasing P-amyrin synthase expression. But salt treatment decreased glycyrrhizic acid and liquiritin ansolute content. That was mainly relative with the significantly decrease of root dry weight, ash and total sugar contents.