Vine Tea Flavonoids And Dihydro Myricetin The Extraction And Purification

Vine tea, whose science name is Ampelopsis Grossedentata, belongs to the Ampelopsis in the VitaceaeMiehx family of wild liane. Ampelopsis Grossedentata, sweet and light in taste, cool in nature, has the effects of clearing away heat and toxic material, relieving rheumatism, and strengthening the bones and muscles. Even the whole plant can be taken for medical uses. When one gets a cold or has a fever or a sore throat, it is always applied. So is in the treatment of hepatitis with jaundice or furuncles.Studies of its chemical elements prove that in Ampelopsis Grossedentata the flavones amount to more than 30%, while dihydromyricetin can achieve 20%.The total flavone extracted from the roots and stems of Ampelopsis Grossedentata enjoy functions of antithrombus,lipid-lowering, liver protection, blood sugar regulating, anti-inflammation, pain easing and enhancing immunity. Dihydromyricetin as the most important one in the flavonoids, is reported to have effect in anti-inflammation, stopping cough, eliminating phlegm, easing pain, preventing bacteria, reducing high blood pressure, regulating blood lipid, detoxification of alcoholism and so on. Thus, the total flavone and dihydromyricetin have a prospect of wide application in foods, health products and medicine.Until now, we have not seen any report about the Ampelopsis Grossedentata's commercialized products in the further processing. Choosing and establishing extraction, isolation and purification technologies of total flavone and dihydromyricetin in Ampelopsis Grossedentata is closely related to the deep development and application of its resources. Based on previous research, this thesis makes the following researches.1. Establishing the method of determining quantities of total flavone and DMY in Ampelopsis Grossedentata.Take dihydromyricetin as control article, adopt ultraviolet spectrophotometry to wavelength scan by dihydromyricetin control article in the 200-800nm and recognize 290 nm is a maximum absorption W/L. By consideration the assay method of addition 5% A1C13 alcoholic solution,2%ZrOCCl2 alcoholic solution and unaddition chromogenic reagent, find the three methods'result is almost the same, so deploy the way of unaddition chromogenic reagent. Take dihydromyricetin as control article to determine contents of dihydromyricetin in vine tea. The chromato bar genealogy is sinochrom ODS-BP(250mm X 4.6mm,5μm); moving phase is Colonial spirit-water-H3PO4(27:73:0.1); flow number is 1.0mL·minute; sample size is 20μL; column temperature is 25℃; W/L is 290nm2. Making clear about the quantities differences of fresh Ampelopsis Grossedentata from different areas and under different processing.Consideration the way of yin heavenly terms,open-air drying,50 ℃airing,70℃airing,90℃airing,110℃airing and 130℃airing to effect total flavone and dihydromyricetin in Ampelopsis Grossedentata. Definite the best way is 70℃airing.3. Optimating the extraction techniques of total flavoneBy the crosscut experimentation,in the reference indicatrix of total flavone extraction percentage,dihydromyricetin extraction percentage,total flavone contents and dihydromyricetin contents to pick out the best abstraction artwork:material ratio is 1:10; alcohol density is 70%; abstraction temperature is 70℃; abstraction times is two. The study provid capital theory parameter for Chinese materia medica gallipots dynamic state countercrrent distribution.4. Researching purification methods of total flavoneConsideration the effect of Colonial spirit,alcohol,acetone,water,the dosage of the best solvent,the dosage of absorbite to purity of total flavone,yield of total flavone,purity of dihydromyricetin,yield of dihydromyricetin to pick out the best and find purification artwork is that water amount is ninty time; activated carbon amount is 1%; temperature is 0-4℃; set time is 24h, by this way 10 times,the dihydromyricetin purity can reach 98%.5. Studying the macroporous resins'enrichment of total flavone from recrystallized mother solutions.By quiet adsorbing to considerat the effect of macroporous ads-orptive resins HPD-100,NKA-9,AB-8,D-101 to total flavonoids' adsorb and desorb. Find he best macroporous adsorptive resins are HPD-100. considerat the dynamic state parameter,scaling-up and periodic duty of HPD-100, the result is that macroporous adsorptiveresins HPD-100 can enrich total flavonoids and the artwork is hare-cryst mother liquid'PH=3; flow number is 2BV/h; the adsorbing breakthrough of Collumn 1 is 6BV, adsorb the amount of recryst mother liquor is 20BV; after the Collumn 2 adsorb the transudate of Collumn 1, adsorb 12BV recryst mother liquid; after the Collumn3adsorb the transudate of Collumn 2, adsorb 12BV recryst mother li-quid; the anagenetic Collumn 1 adsorb the transudate of Collumn3,the 3 roots balsam Collumn to compose a cascade system, by this way to circulate manipulate or operate. each balsam Collumn adsorb 30 minute, before use the distilled water elute to see the liquoris achromasy. afte that, use 70% alcohol of 40ml/g(dry balsam) to elute, collect the alcohol elutriant, reclaim alcohol, aridity, to obtain total flavonoids of macroporous resin enrich,which is iteration recryst purify by the water.