Mir-200 Family In Human Gastric Cancer Tissues And Their Biological Behavior Of Gastric Cancer Cells Research

OBJECTIVE:To study differently expression of miR-200 cluster in gastric carcinoma and pericarcinoma tissure. And to investigate the relativity of differently expression between miR-200 cluster and E-cadherin.METHODS:We used 2- (△△Ct) RT-PCR to detect miR-200a, miR-200b, miR-200c, miR-141, miR-42, E-cadherin, in 25 cases of gastric carcinoma and pericarcinoma tissure .RESULTS:Compared to pericarcinoma tissure,the expression of miR-200 cluster was statistically significant difference down-regulated miR-200a(76%),miR-200b(88%), miR-200c(84%)miR-141(88%)miR-429(76%) ,and as well of E-cadherin mRNA(P<0.01)in all cases of gastric carcinoma.CONCLUSION:There is positive correlation between miRNA-200 cluster expression and E-cadherin, expression, and probably based on gastric carcinoma invasion and migration. The results of thisstudy lay the foundation for further studying on the role of miR-200 cluster in gastric cancer. OBJECTIVE:To investigate the effect of overexpression of miR-200b and miR-200c in apoptosis, proliferation and invasion of MKN-28 cell function; and to clarify the effect of miR -200 in tumor evolution.METHODS:To upregulate expression of miR-200 into MKN-28 cell, transfection of miR-200b mimics and miR-200c mimics was used; then detected apoptosis and cell proliferation change of MKN-28 by FCM(flow cytometry); and detected invasion change of MNK-28 by transwell assay and cell scratch testRESULTS:Overexpression of miR-200 by transfetion of miR-200b mimics and miR-200c mimics enhanced apotosis of MNK-28, certify apoptosis function of miR-200 in gastric cancer. Overexpression of miR-200b and miR-200c suppressed cell proliferation of MNK-28. Transfetion of miR-200b mimics and miR-200c mimics enhanced the invasion of MNK-28, there is signification difference when compared to control; we did not view change of cell migration in MNK-28 after tansfection.CONCLUSION:Disorder of miR-200b and miR-200c expression could result in biology function change in gastric cancer cell, which, including enhance of cell apoptosis, suppression of cell proliferation and change of cell invasion. Clarification that miR-200 was important in gastric cancer evolution. Experssion of miR-200 was achievable by heteroinfection, this in turn be a possible way to intervene tumor cell and a novel way of oncotherapy.