Geto Extract Appv717i Transgenic Mouse Pp2a Expression In Hippocampal Neurons

Objective:To investigate the effect of GEPT extract on expression of protein phosphatase 2A (PP2A) in hippocampal neurons of APPV717I transgenic mice.Methods:60 three-month-old APPV717I transgenic mice were randomly divided into model group, donepezil group (0.92mg/kg/d), GEPT low-dose group (0.075g/kg/d), GEPT medium-dose group(0.075g/kg/d) and GEPT high-dose group (0.075g/kg/d), and 12 three-month-old C57BL/6J mice of the same genetic background were set as normal control group, the treatment groups were administered oral ly 1 time a day, and both the control group and model group were given 0.5% sodium carboxymethylcellulose (CMC) solution. Immunohistochemistry and Western blot analysis were used to detect the expression of protein phosphatase 2A (PP2A) in hippocampal neurons of APPV717I transgenic mice after 8 months drug administration (11 months old).Results:Immunohistochemical staining of PP2A in hippocampal CA1 region neurons of the model group were thin, and the number of positive cells and the total area of positive cells were significantly decreased compared with that of the control group (P<0.01); the number of pos i t ive cells and the total area of positive cells of the GEPT low, medium and high-dose group were significantly increased compared with that of the model group (P<0.01); the number of positive cells and the total area of positive cells of the GEPT low-dose group showed significant difference compared with that of the control group (P<0.05, P<0.01); the number of positive cells and the total area of positive cells of the GEPT medium and high-dose group showed no significant difference compared with that of the control group (P>0.05), and the effectiveness were similar compared with that of the donepezil group.Western blot analysis of PP2A expression in hippocampal neurons of the model group were significantly decreased compared with that of the control group (P<0.01); the PP2A expression of the GEPT low, medium and high-dose group were significantly increased compared with that of the model group (P<0.01); the PP2A expression of the GEPT low-dose group showed significant difference compared with that of the control group (P<0.05); the PP2A expression of the GEPT medium and high-dose group showed no significant difference compared with that of the control group (P>0.05), and the effectiveness were similar compared with that of the donepezil group. The results of Western blot analysis were similar to that of Immunohistochemistry.Conclusion:GEPT can activate the expression of PP2A in hippocampal neurons of APPV717I transgenic mice, which prompt GEPT may attenuate abnormal hyperphosphorylation of tau protein in hippocampal neurons of APPV717I transgenic mice through this way.