The Impact Of Massage On The Sciatic Nerve Injury After Ntfs

[Objective]This study is based on the good clinical effect of tuina in peripheral nerve injuries, via experiments to explore the mechanism of tuina treatment in peripheral nerve injuries. Through analyzing the behavior and morphology of rats, analyzing nerve growth factor(NGF),ciliary neurotrophic factor(CNTF), braind-deried neurotrophic factor(BDNF), basic fibroblast growth factor(bFGF), to research the mechanism of tuina treatment in peripheral nerve injuries.[Method]This topic is based on past experiments, SD rats are choosed as the experimental animal, using the model of sciatic nerve with crush injury, using "tuina technique simulator" to stimulate rat's right side(surgical side) yinmen chengshan and yanglingquan, which executes pointing poking and keading technique. Through analyzing the inclined plane test and pain threshold and gastrocnemius weight, to explore the recovery effect of body obstacles; observing gastrocnemius HE dyed by microscope, to explore tuina effects of muscular dystrophy; observing sciatic nerve HE dyed by microscope, to explore tuina's prtective effects of neuron; by analyzing Elisa test in gastrocnemius and in blood, and by analyzing immunohistochemistry in dorsal horn, to find NGF CNTF BDNF bFGF changes, in order to tuina affective in those neurotrophin.[Results]1 Inclined Plane TestResult shows that tuina therapy group is differ from model group. It verifies that tuina therapy is effective for recovering muscle power after rat's sciatic injury.2 Pain ThresholdResult shows that tuina therapy group is differ from model group. It verifies that tuina treatment is effective for rat's sensorimotor recovery.3 Gastrocnemius WeightsRat's gastrocnemius weights lose obviously. There are no differences between Tuina therapy group and model group.4 Gastrocnemius HE dyedThe normal group shows that muscle cells are full, muscle cell and nucleus distributed homogeneously, fibromuscular gap is normal.The Model group shows that fibromuscular gap is bigger and muscle cells atrophied Nuclei is increased. The Model control group shows that fibromuscular gap is bigger but less than model group, and muscle cells atrophied Nuclei is increased.The Tuina therapy group shows that fibromuscular gap is bigger but less than model group and model control group, muscle cells atrophied.5 Sciatic nerve HE dyedThe normal group shows that Axon and schwann cells arranged formally.The model group shows that axon is swelling, Axon and schwann cells bursted which arranged mess, Glial cells is increased obviously.The Model control group is same to the model group.The tuina therapy group is same to the normal group.6 NGF,CNTF,BDNF,bFGF Elisa TestThe results show that NGF CNTF BDNF contents in gastrocnemius are higher than in bloods, bFGF content in gastrocnemius is lower than in bloods.NGF result shows that comparing between plasma has no differences P>0.05. comparing between Gastrocnemius:model group has differences with others P<0.05.CNTF result shows that comparing between plasma:tuina therapy group with model control group have differences P<0.05. comparing between Gastrocnemius:model group with normal group and model control group have differences P<0.05.BDNF result shows that comparing between plasma:normal group model control group and tuina therapy group with model group have differences P<0.05. comparing between Gastrocnemius:normal group model control group and tuina group with model group have differences P<0.05.bFGF result shows that comparing between plasma:model group and model control group with normal group have differences P<0.05. comparing between Gastrocnemius:model group and model control group with normal group have differences P<0.05.7 Immunohistochemistry(IHC)IHC result shows that NGF,bFGF,BDNF,CNTF are high expression in the tuina therapy group.NGF comparing between groups show model control group with normal group model group and tuina treatment group have differences P<0.05.CNTF comparing between groups show tuina treatment group with normal group model group and model collector group have differences P<0.05.BDNF comparing between groups show normal group model group and model control group with tuina treatment group have differences P<0.05. bFGF comparing between groups show normal group model group and model collector group with tuina treatment group have differences P<0.05.[Conclusion]1 After sciatic nerve injuries, tuina treatment is effective in rats muscle power and pain feeling recovery.2 Nerves cell in sciatic in Tuina therapy group recoveries good, it tells tuina treatment is effective for sciatic nerve injuries.3 According to the results of Gastrocnemius weight shows that tuina treatment not effective yet for muscular dystrophy in 10 times treatments.4 Tuina treatment affects NGF,bFGF,BDNF,CNTF to recovery nerve injuries,it protects neuron, and it rebuilts nerve axoplasmic transportation pathway.