Allicin On Rabbits With Acute Lower Limb Ischemia - Reperfusion Injury After Il-1, Il-6, Il-8 Content Of The Impact

Objective: To observe the impact and significance of allicin injection on rabbits with acute limb ischemia reperfusion tissue by IL-1,IL-6,IL-8 content.Methods :30 rabbits were randomly divided into 5 groups:control group, ischemia-reperfusion 2hours group, ischemia-reperfusion 5 hours group, ischemia-reperfusion 2 hours with allicin treatment group, ischemia-reperfusion 5 hours with allicin treatment group..Control group,cutting limbs with saline drops and taking gastrocnemius.The remaining four groups:we copying ischemia-reperfusion model and made allicine or saline drops in the ear vein 10 minutes before the deadline of ischemia 2 or 5 hours groups,taking gastrocnemius after ischemia-reperfusion for 1 hour as immune parameters measurement and comparative analysis of morphological observation.Results :IL-1,IL-6,IL-8 content in tissues of ischemia-reperfusion 2 hours group and 5 hours group were significantly higher than that of control group(P<0.05). IL-1,IL-6,IL-8 content in tissues of ischemia-reperfusion 2 hours with allicin treatment group and ischemia-reperfusion 5 hours with allicin treatment group were significantly lower than that of ischemia-reperfusion 2 hours group and 5 hours group(P<0.05).The physiological changes of tissues structure was much more obvious with both the ischemia-reperfusion group but less obvious with both the treatment group at light microscope.Conclusion :Allicin may inhibit the expression of IL-1,IL-6,IL-8 on rabbits with acute limb ischemia reperfusion tissue,reducing neutrophil adhesion and infiltration together with the inflammatory exudate effectively,inhibit the permeability of microcirculation,reduce the muscle injury of lower limb.