Simple Herpes Virus Type 1 Envelope Glycoprotein G, The Prokaryotic Expression Clone Construction, Expression And Initial Purification,

For serodiagnosis of acute virus infections, 1gM-specific serologic detection has been proven to be valuable. However, all of the available 1gM assays suffer from limited specificity and sensitivity for diagnosis of HSV- 1 infection, the main reason is that antigens used now is an only poorly defined extracts of virus antigens purified from extracellular particles. Because of the poor specificity and purification as well as different components of the used antigens without common standard, the results are not satisfying. It has been shown that single specific protein or polypeptide, produced via engineering or chemical synthesis, can be used as good antigen in serological diagnosis. This makes it possible to obtain HSV- 1 specific antigen with artificial technique. In this research, an epitope gene of HSV- 1 Us4 was amplified by PCR, both the target gene and expression vector were digested by double enzyme and ligated, the recombinant plasmid was transfered into Ecoli.BL2L and induced by IPTG. The product was purified by glutathione-sepharose affinity chromatography, identified by SDS-PAGE and Western-Blot. It has shown that the recombinant protein has strong antigenic characterization, it could identify 80% acute HSV- 1 infection serum. This study will improve the serodiagnosis for acute HSV- 1 infection.