Maternal Blood Of Fetal Nucleated Red Blood Cells Enrichment

Objective To isolate feta~ nucleated red blood cells (}7NRBCs) from maternal blood ,which be used in noninvasive prenatal genetic diagnosis Methods Peripheral blood mononuclear cells (MNCs) were seqarated by single density gradient Histopaque-1077 from 32 pregnat women with gestations behve~n 1 b2C) weeks ,FNRJ3Cs were entiched from the M7NCs by flow cytometiy using monoclonal antibody against the glvcophotin A(GPA).Sortecl cells of male fetuses in maternal blood were con県med usim i-special DNA sequences (SRi) by polytnerase chain reaction ( PCR)and by Y梒liiomosotne fluorescent staining. Results (193 ? i0~?. ii X I (Y3GPA positive cells per milliliter in pregnant women pcaipheral blood were softe(l by the flow cytornetry.More 90% results of PCR were identical with the g~n~kr dkr ahorticide. Conclusioit The FNRI3Cs in mak~imd blood were punhW 憕nd enriched to some degree by density gradient and flow eytometrv (lie relation b~t~~e~n the numbers of EN~RDCs and sonic weeks ot pte~ice are no prcscnt,to ;tnak~. I . t,n抐atioti for noniuvasivc. prenatal QdneIic. diaonosts.■END-END Key words Fetal Nucleated red blood cell;Density gradicnt cenuifuge;Flow cytometry;PCR...