Pediatric Compound Paracetamol And Amantadine Hydrochloride Suppository Experimental Study

Objective: 1. To study antiviral and antipyretic analgesic actions of Paracetamol and Amantadine Suppositories (PAS) .2. During study of pharmacokinetics of paracetamol, a HPLC for determination of paracetamol in rabbit was developed.Methods: 1. The cell culture was used in extrinsic ar抰iviral experiment; others were mouse influenzal viral preumonia experiment, rabbit bacterial toxin fever experiment, and the anti-inflammatory experiment and analgesic experiment caused by I-JAc, and LD50 dose is 748mg/kg.2.HP1 100 system was adopted with a DAD detector, a ODS 100 C18 column (5 ii m,250mmx4mm), a mobile phase consisted of 70% water and 30% methanol and using a flow rate of 1 .Oml/min. After deproteinized with acetonitrile, (PAS) sample was directly injected.Results: 1. PAS could greatly raise the survive time and rate of mice infected by influenzal virus, and possessed obvious antagonize against influenzal virus, and alleviated the pulmonary pathologic change of mice induced influenzal viral pneumonia, and decreased the lung indexes of influenzal virus A; PAS could greatly shrink rabbit fever induced by bacteria toxin, and raise the pain threshold value caused by heat and H~ stimulus, and has no obvious effect to Evans blue exudates cause by HAc and peritonitis.2.The method have a minimum detection limit of 0.05~tg/ml, after single oral administration of 48mg/kg of Paracetamol capsule and suppository the disposition was conformed to a first order absorption a one- 梒ompartment model. The pharmacokinetic parameters of PAS were as follows: T1/2Ka 0.115h?and 0.08h1, T~ 0.42h1 and 0.15h71, Cmax 4.49pg/ml and 48.6~~tg/ml, AUC 11 .26j.tglmbh and 38.1 pg/mFh respectively.Conclusion: The result shows that a simple sensitive method for HPLC determination of Paracetamol in rabbit was developed, and Paracetamol suppository was absorbed and reached peak-time quickly.