Antisense Wire Mitogen Activated Protein Kinase Nucleotide Proliferating Cell Nuclear Antigen Gene Expression In Vascular Smooth Muscle Cells

The proliferation of vascular smooth muscle cells(VSMC) play important role in the initiation and development of artherosclerosis. The mitogen-activated protein kinase(MAPK) activated in response to a variety of extracellular stimuli can induce the proliferation of VSMC. Proliferating cell nuclear antigen (PCNA) can be considered as a direct and dependable indication of cell proliferation. Antisense oligonucleotides can selectively inhibit certain gene expression so that obstacle the progress of pathophysiology related to that gene. It is uncertain whether the antisense MAPK oligonucleotides can inhibit the proliferation of vascular smooth muscle cells. In this study, we investigate the influence of antisense MAPK oligonucleotides on the PCNA gene expression, in order to know whether it can inhibit the proliferation of VSMC , which should provide a theoretical basis for clinical use.Objective: To observe the influence of antisense MAPK oligonucleotides on the PCNA gene expression, so that to know whether it can inhibit the proliferation of VSMC or not.Methods: (1) Establish the model of cultured cell. The VSMC were identified by immuno-fluorescent staining. (2) Cultured rat VSMC were treated with the antisense oligonucleotides of mitogen -activated protein kinase, then northern blot hybridization analysis was performed to determine the expression of PCNA mRNA.Results: After 72h treatment with 0.8 (mol/L antisense MAPK oligonucleotides, the expression of PCNA mRNA of VSMC was remarkably inhibited compared to the control (treated with 20%FBS).Conclusion: The antisense oligonucleotides of mitogen -activated protein kinase can inhibit the proliferation of cultured VSMC through downregulating the PCNA mRNA.