Secondary Lymphoid Tissue Chemokine (slc) Cloning And Expression In Pichia Pastoris System

With PCR and molecular clone technique, we amplified the SLC gene which was selected from the human lung,s cDNA library and inserted it into the Pichia pastoris expression vector PPIC9. Linerized PIC9K/SLC was transformed into the Pichia pastoris GS115. Checking with PCR, the sequence of SLC was correctly confirmed. Taking advantage of the G418 enabled us to select the most efficient bacteria.After methanol induction, the result of SDS-PAGE showed the expression of the protein of SLC and its molecular weight about 18KD.Based on the activity assay, the SLC purified by us has been confirmed the good activity for lymphocyte chemotaxis.