Ala Pdt In Vitro Kill Leukemia Cancer Cells New Method

Photodynamic therapy, abbreviated to PDT, is a therapy which uses the photodynamic or actinic chemistry interrelation occurred in the cells. We introduce the research status about the PDT which takes place domesticly and overseas, and expatiate on the significance of our research and the content of our research at the first part of the dissertation. In the second part, the basic pathology of leucocythemia has been expatiated simply, and the conventionally therapies that have been currently applied in the treatment of leucocythemia are summarized. Followed, in the third part, we detailedly dissertate the principle of PDT > the photosensitizer and the lamp-house used in the course of PDT. In the forth part, it is introduced the experiment system of ALA-PDT which we established. In the fifth part, by use of the experiment system of ALA-PDT, we research the leukaemia cells - HL60 and achieve a serial of optimizational experiment parameters. In the last part, the whole dissertation is summed up.The major achievements made in this dissertation are as follows: 1 . The basic pathology of leucocythemia has been summarized and the conventionally therapy of leucocythemia was fully introduced.2. We probe deeply into the action mechanism of ALA-PDT and set forth on light-dose, light- wave used in the course of PDT and the noxious side-effect followed in the treatment of ALA-PDT.3. The author designed the light-reaction room of ALA-PDT and set up the whole PDT experiment system, which uses a common lamp-house and can be adjusted to choose multiwave bands from 400nm to 650nm and be output kinds of power below 8mW.4. By use of the experiment system of ALA-PDT, we research the leukaemia cells - HL60 and achieve a serial of optimizationalabstractexperiment parameters, such as: the density of ALA, the culture time of ALA , the shined-light dose , the shined-light power , the shined-light wavelength and the density of PCS in the course of cell culture.