Hematoporphyrin Monomethyl Ether - Photodynamic Therapy Induced Apoptosis In Hela Cells Inquiry Into The Mechanism

Photodynamic therapy (PDT) is a promising tumor treaty and attracts many countries' attention. For the purpose of further clinical application, we study the HeLa apoptosis induced by HMME-PDT using the hematoporphyrin monomethyl ether (HMME), which is the 2rd photosensitizer developed by our country.In this paper, the base information of PDT, the mechanism of ablating tumor and the signal transduction are introduced firstly. Then, we study the effect of PDT on apoptosis. According to the former research, flow cytometry (FCM) is used to examine apoptosis induced by the optional photosensitizer concentration and illumination. Calcium ion often benefits to apoptosis. With the help of molecular probe and the chelate of calcium ion, we define the effect of calcium ion on apoptosis and evaluate the ERKl/2's role on apoptosis by means of PD98059. Finally, the role of PDT on Bcl-2 is defined by western blot. The main conclusions are listed in the following:1. HMME-PDT can induce HeLa cell apoptosis.2. There is a calcium oscillation after HMME-PDT. In the case of low laser power, increasing intracellular calcium ion concentration ([Ca2+]i) to certain extent can rescue cell from apoptosis. But this does not work in the case of high laser power, i.e., increasing [Ca2+], does not affect the cell apoptosis when the laser power is higher.3. In the case of low laser power, HMME-PDT can activate ERK1/2 signal transduction pathway, which stimulates cell to proliferate. However, in higher power, ERK1/2 may promote cell to apoptosis.4. HMME-PDT has no significant effect on the content of Bcl-2.