Andrographolide New Drug Loading Anti-inflammatory Effects And Its Mechanism

This article is to study and research the anti-inflammatory action and mechanisms ofandrographolide W. By contrasting to andrographolide pellet, to appraisal the effect andauthenticate the ascendancy of andrographolide W. By researching the action ofandrographolide W to mediators of inflammation, explain the potential anti-inflammatorymechanisms.1 The anti-inflammatory actions of Andrographolide W and pellet to different models Researching the experimentation of albumen leaded forced foot in rats, discovered thatall three doses of W can inhibit the swelling degree. Andrographolide W can produce effectjust 30min after administration and the effection can continue more than 8 hours. Contrastto the control group, W have the distinguished effection (P<0.01). Andrographolide pellethas anti-inflammatory action (contrast to control group P<0.01), but the action is muchinferior than that of andrographolide W. The above conclusions explain that W can inhibitthe local swelling degree during inflammatory. Researching the experimentation of xylene leaded forced ear in mice, discovered thatandrographolide W has market anti-inflammatory action, contrast with control group, theeffect of anti-inflammatory action displays evident difference(P<0.01). However,andrographolide pellet has almost no effect in this model(P>0.05). Explaining thatandrographolide W can inhibit the intensification of capillary permeability. The experiment of HAC leaded capillary permeability intension in mice indicate: Wcan inhibit the intension of capillary permeability leaded by HAC obviously, contrast tocontrol group, P<0.01. Andrographolide pellet can also inhibit the intension, but the degreeof inbibition is much lower than W. This research is also explains that andrographolide Whas strong effect of inhibition the capillary permeability intensification. The experiment of granuloma leaded by planted cotton ball in mice can arrive thefollowing conclusion: andrographolide W has strong effect of inhibiting the hyperplasia,the middle dose grop has the most perfect action, the degree of inhibition arrive to 30.99%,the degree of inhibition of small and big doses each are 23.84% and 23.47%. Theinhibition ratio of andrographolide pellet is worst, just 16.39%. The above conclusionexplains that W can inhibit the chronic inflammation in a distance.2 The anti-inflammatory actions of Andrographolide W and pellet to CAR leaded inflammation In this research we observe the diffusate amout and the WBC amout, pr content indiffusate. We get the following conclusion: andrographolide W can obviously inhibit thediffusate amout. Contrast to the control group, the small group P<0.05, the effects ofmiddle and big dose groups are much obvious, P<0.01. In this conclusion, we can alsodiscover that to the inhibit effect of diffusate, W is weaker than Asp group but strongerthan andrographolide pellet group. To the WBC amout in inflammatory, andrographolideW displays perfect inhibit action. Contrast with the control group, the difference is obvious.Among them, the middle dose P<0.05, the big dose P<0.01. Contrast to theandrographolide pellet group, the effect of W is a little better. To the content of pr indiffusate, the middle and big dose groups display obvious effection, P<0.01. The smalldose and pellet groups also have inhibit effection, P<0.05. Contrast to the pellet group, theactions of middle group and big group are better.3 The approach to the anti-inflammatory mechanisms of andrographolide W Determining the amount of PGE2, LTB4, TNF-α,IL-1β,IL-6,IL-8 by immunitymeans, we get the following conclusions: The conclusion of the researching of PGE2 and LTB4 display: the effection to PGE2 isstronger than to LTB4, all the three doses of andrographolide W can degrade the content ofPGE2 in diffusate, P<0.01. To the influence to LTB4, the small dose of W contrast with thecontrol group has no obvious difference, the middle and big groups can degrade theamount of LTB4 in diffusate (P<0.01).The conclusion of the research of cell factors display: andrographolide W has the besteff...