Pdgfra Expression In Human Neural Tube Defects In Embryos

Objective Screen differentially expressed genes of neural tube defects (NTDs) by cDNA microarray technique among the population of Shanxi Luliang. Classify the target genes to different pathways, such as metabolism, gene translation regulator, signaling, apoptosis and so on. Select the platelet-derived growth factor receptor-alpha PDGFR α ) and it's regulators(PAX1, PAX3-FKHR, EGFR)to study further.Method Using high-throughput genechip technology to analysis the expression pattern of NTDs. All these target genes were classified to different pathways, such as metabolism, gene translation regulator, signaling, apoptosis and so on. We select the platelet-derived growth factor receptor-alpha (PDGFRα) as our target gene. PAX1, PAX3-FKHR ,EGFR as the gene's regulators were also chosen to test the change of PDGFRα in NTDs embryos of different weeks. The immunohistochemical was used to verify the protein's expression of PDGFRα . Results we obtain 22209 differentially expressed genes to human NTDs. There were 74 genes over-expressed about 2 times ,they were 3 genes of oxidative phosphorylation,7genes of zinc finger,2 genes of glutathione metabolism,2 genes of eicosanoid synthesis,4 genes of matrix metalloproteinases, 6 genes of G protein signaling pathway, and there were 387 genes low-expressed about 2 times ,they were 8 genes of DNA replication and nucleotide metabolism,7 genes of inflammatory response pathway, 6 genes of apoptosis,2 genes of Integrin-mediated cell adhesion,4 genes of citrate cycle, 9 genes of G protein signaling pathway and so on. PDGFRα is our target gene, it shows over-expression about 2^0.9 times.The RT-PCR(p<0.01) and immunohistochemical(p<0.05) also show the same results. Conclusions: The genes 'expression in NTDs has great difference with controls. It prove that the development of NTDs is a complicated course at different stages, The NTDs may results from the reason that many kinds of genes' abnormal-expression including DNA replication and nucleotide metabolism, signaling pathway , apoptosis et al; PDGFRa has been shown to regulate cell growth and survival, contribute to cell...