Bone Marrow Stromal Cell Transplantation Used Along With Brain-derived Neurotrophic Factor To Promote The Repair Of Spinal Cord Injury In Experimental Research

Objective: To study the effect of exogenous BDNF combined with bonemarrow stromal cells (BMSCs)inpromoting axon recovery after spinal cordinjury(SCI) in adult rats. Methods:BMSCs were obtaind from rats and werelabeled by BrdU; Wister rats with T12 spinal cord hemisection were divided into4 groups:control group (DMEM group), BDNF group, BMSCs transplantationgroup (BMSCs group) and BDNF+BMSCs group. Animals were behaviorallytested for 8 weeks using the inclined plane test and modified Tarlov locomotorrating scale. After 2 months pathological changes of spinal cord tissues wereobserved with light and electronic microscopy. Immunocytochemical stainingwas conducted for BrdU, neurofilament(NF), glial fibrillary acidicprotein(GFAP) and the result analyzed with image pattern analysis. Results:BrdU reactive cells were found in spinal cord microenvironment withimmunohistochemical staining. These grafts can be well integrated with thehost and survive. The rats implanted with BMSCs showed remarkablerecovery of the voluntary locomotive function of hind limbs. Rats treated withBDNF+BMSCs had more improvements in hind limb function than treated withBMSCs or BDNF alone. Conclusion: Exogenous BDNF and purified BMSCscan promote long descending system regeneration and prevent neuralatrophy,partially mediating the recovery of hind limb function spinal cord injury. Theremay be a synergistic effect between BDNF and BMSCs.