As <sub> 2 </ Sub> O <sub> 3 </ Sub> Inhibition Of Human Breast Cancer Xenografts In Nude Mice Lymphangiogenesis Experimental Study

Objective: To investigate the morphological characteristic of lymphatics and to reason the intratumoral lymphangiogenesis in transplantation tumor model for human breast infiltrating duct carcinoma in nude mice. Methods: Human breast carcinoma tissue mass were transplanted into 5 nude mice to develop implanted tumors. HE staining was used to observe the development of these tumors; VEGFR-3 label staining was performed to observe lymphatics,and ultrastructure of lymphatics was observed with transmission electron microscopy . Results: Tumor occurred in a11 the mice, and grew up to about 0.6~ 0.8 centimeters after 52 days when specimen was collected. Under light microscope with immunohistochemistry, there were some lymphatics in all the tumors, and probably there in periphery was much more than in the center of these tumors. Under TEM some characteristic of neonatal lymphatics can be seen, such as thick dissepiment but narrow cavum, changes of organell, and so on. Conclusions: Neonatal lymphatics can be seen in transplantation tumor model for human breast infiltrating duct carcinoma in nude mice.